〒819-0395 福岡市西区元岡744 ウエスト5号館 530号室
092-802-4570
kusakabe(at)agr.kyushu-u.ac.jp (at)→@

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昆虫ゲノム科学研究室

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発表論文(2015年以降)

2021年 2020年 2019年 2018年 2017年 2016年 2015年

国際学会での発表(2015年以降)

2020年 2019年 2018年 2017年 2016年 2015年

発表論文(2015年以降)

2021年8月現在 6本

Tsubokawa D., Kikuchi T., Lee J.M. Kusakabe T., Yamamoto Y., Maruyama H.
Venestatin from parasitic helminths interferes with receptor for advanced glycation end products (RAGE)-mediated immune responses to promote larval migration
https://doi.org/10.1371/journal.ppat.100964

Takahashi M., Tehseen M., Salunke R., Takahashi E., Mfarrej S., Sobhy M.A., Alhamlan F.S., Hala S., Ramos-Mandujano G., Al-Qahtani A.A., Alofi F.S., Alsomali A., Hashem A.M., Khogeer A., Almontashiri N.A.M., Lee J.M., Mon H., Sakashita K., Li M., Kusakabe T., Pain A., and Hamda S.M.
Quick and Easy Assembly of a One-Step qRT-PCR Kit for COVID-19 Diagnostics Using In-House Enzymes
https://doi.org/10.1021/acsomega.0c05635

Ebihara T., Masuda A., Takahashi D., Hino M., Mon H., Kakino K., Fujii T., Fujita R., Ueda T., Lee JM., Kuskabe T.
Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System
Molecular Biotechnology
https://doi.org/10.1007/s12033-021-00373-0

Musuda A,, Lee JM., Miyata T., Ebihara T., Kakino K., Hino M., Fujitya R., Mon H., Kusakabe T.
Stable trimer formation of spike protein from porcine epidemic diarrhea virus improves the efficiency of secretory production in silkworms and induces neutralizing antibodies in mice
Veterinary Research
https://doi.org/10.1186/s13567-021-00971-5

Ebihara T., Xu J., Tonooka Y., Nagasato., Kakino K., Masuda., Minamihata K., Kamiya N., Nakatake H., Chieda Y., Mon H., Fujii T., Kusakabe T., Lee J.M.
Active human and murine tumor necrosis factor α cytokines produced from silkworm baculovirus expression system
Insects
https://doi.org/10.3390/insects12060517

Nagai R., Ebihara T., Kakino K., Masuda A., Xu J., Minamihata K., Kamiya N., Kongkrongtong T., Kawahara M., Mon H., Fujii T., Kusakabe T., Lee J.M.
Production of an active Mus musculus IL-3 using updated silkworm-based baculovirus expression vector system
Journal of Asia-Pacific Entomology
https://doi.org/10.1016/j.aspen.2021.04.011


2020年 7本

Fujii T., Kakino K., Tanaka M., Lee J.M., Kusakabe T., Banno Y.
A defect in purine nucleotide metabolism in the silkworm, Bombyx mori, causes a translucent larval integument and male infertility
Insect Biochemistry and molecular Biology
https://doi.org/10.1016/j.ibmb.2020.103458

AI-Amoodi A.S., Sakashita K., Ali A.J., Zhou R., Lee J.M., Tehseen M., Li M., Belmonte J.C.I., Kusakabe T., and Merzaban J.S. (2020)
Using Eukaryotic Expression Systems to Generate Human α1,3-Fucosyltransferases That Effectively Create Selectin-Binding Glycans on Stem Cells
Biochemistry
https://doi.org/10.1021/acs.biochem.0c00523

Fujita R., Hino M., Ebihara T., Nagasato T., Masuda A., Lee JM., Fujii T., Mon H., Kakino K., Nagai R., Tanaka M., Tonooka Y, Moriyama T., Kusakabe T. (2020)
Efficient production of recombinant SARS-Cov-2 Spike Protein using the baculovirus-silkworm system
BBRC
https://doi.org/10.1016/j.bbrc.2020.06.020

Kakino K., Masuda A., Hino M., Ebihara T., Xu J., Mon H., Fujita R., Fujii T., Kusakabe T., Lee JM. (2020)
Efficient production of recombinant T7 endonuclease I using silkworm-baculovirus expression vector system
J. Asia-Pacific. Entomol.
https://doi.org/10.1016/j.aspen.2020.05.001

Aleisa F., Sakashita K., Lee JM., AbuSamra D., Alwan D., Nozue S., Tehseen M., Hamdan S., Habuchi S., Kusakabe T. and MerzabanJ. (2020)
Functional Binding of E-selectin to its Ligands is Enhanced by Structural Features Beyond its Lectin Domain
J. Biol. Chem.
http://www.jbc.org/cgi/doi/10.1074/jbc.RA119.010910

Kobayashi M., Xu j., Kakino K., Masuda A., Hino M., Fujimoto N., Minamihata K., Kamiya N., Mon H., Iida H., Takahashi M., Kusakabe T., Lee JM. (2020)
Optimal silkworm larva host for high-level production of Mus musculus IL-4 using a baculovirus expression vector system
J. Asia-Pacific. Entomol.
https://doi.org/10.1016/j.aspen.2019.12.014

Hino M.†, Karasaki N. †, Tatsuke T., Morokuma D., Masuda A., Mon H., Lee JM, Kusakabe T. (2020)
†Both authors contributed equally to this manuscript
Mild inactivation and inhibition of phenoloxidase in silkworm serum for xeno-free culture of insect cells
J. Insect Biotechnol. and Sericol
https://doi.org/10.11416/jibs.89.1_009


2019年 11本

Bingqian Li, Zhiqing Li , Chenchen Lu, Li Chang, Dongchao Zhao, Guanwang Shen , Takahiro Kusakabe, Qingyou Xia, Ping Zhao (2019)
Heat Shock Cognate 70 Functions as A Chaperone for the Stability of Kinetochore Protein CENP-N in Holocentric Insect Silkworm
Int. J. Mol. Sci. (2019) 20(23), 5823
https://doi.org/10.3390/ijms20235823

Sun Mee Hong., Ji-Hyun Choi., Sun-Jung Jo., Kwan-Sik Min., Dae-Jung Kim., Lee JM., Kusakabe T. (2019)
Heterologous Production and Glycosylation of Japanese Eel Follitropin Using Silkworm
Biotechnol. and Bioprocess Engineering 24: 745-753
https://doi.org/10.1007/s12257-019-0045-2

Xu J., Lee JM., Tatsuke T., Ebihara T., Masuda A., Hino M., Morokuma D., Fujita R., Mon H., Kusakabe T., Takahashi M. (2019)
Expression and Purification of Vaccinia Virus DNA Topoisomerase IB Produced in the Silkworm–Baculovirus Expression System
Mol. Biotechnol. (2019) 61: 622–630
https://doi.org/10.1007/s12033-019-00184-4

Morishita M., Mon H., Lee JM., Kusakabe T., Maximiano Corrêa Cassal, Yasunaga-Aoki C., Iiyama K. (2019)
Toxin complex is a major virulence determinant of Enterobacter sp. 532 against the silkworm, Bombyx mori
J. Insect. Biotechnol. and Sericol. 88(1), 1_021-1_025.
https://doi.org/10.11416/jibs.88.1_021

Tsubokawa D., Lee JM., Hatta T., Mikami F., Maruyama H., Arakawa T., Kusakabe T., Tsuji N. (2019)
Characterization of the RAGE-binding protein, Strongyloides venestatin, produced by the silkworm-baculovirus expression system
Infection, Genetics and Evolution 75(2019) 103964
https://doi.org/10.1016/j.meegid.2019.103964

Yano T., Lee JM., Xu J., Morifuji Y., Masuda A., Hino M., Morokuma D., Fujita R., Takahashi M., Kusakabe T., Mon H. (2019)
Expression of the thermostable Moloney murine leukemia virus reverse transcriptase by silkworm-baculovirus expression system
J. Asia-Pacific. Entomol. 22,2,453-457.
http://doi.org/10.1016/j.aspen.2019.02.008

Kinoshita Y,, Xu J., Masuda A., Minamihata K., Kamiya N., Mon H., Fujita R., Kusakabe T., Lee JM. (2019)
Expression and purification of biologically active human granulocyte-macrophage colony stimulating factor (hGM-CSF) using silkworm-baculovirus expression vector system
Protein Expr. Purif. 159:69-74.
https://doi.org/10.1016/j.pep.2019.03.010

Morio A., Xu J., Masuda A., Kinoshita Y., Hino M., Morokuma D., Hatsumi M.Goda., Okino N., Ito M., Mon H., Fujita R., Kusakabe T., Lee JM. (2019)
Expression, purification, and characterization of highly active endo-α-N-acetylgalactosaminidases expressed by silkworm-baculovirus expression system
J. Asia-Pacific. Entomol. 22,2,404-408.
http://doi.org/10.1016/j.aspen.2019.01.009

Patmawati., Minamihata K., Tatsuke T., Lee JM., Kusakabe T., Kamiya N. (2019)
Functional horseradish peroxidase-streptavidin chimeric proteins prepared using a silkworm-baculovirus expression system for diagnostic purposes
J. Biotechnol. 20;297:28-31.
doi.org/10.1016/j.jbiotec.2019.03.007

Permana D., Minamihata K., Tatsuke T., Lee JM., Kusakabe T., Goto M., Kamiya N. (2019)
Polymerization of horseradish peroxidase by a laccase-catalyzed tyrosine coupling reaction
J. Biotechnol. 27:e1800531.
https://doi.org/10.1002/biot.201800531

Morishita M., Masuda A., Mon H., Lee JM., Kusakabe T., Tashiro K., Yasunaga-Aoki C., Iiyama K. (2019)
Identification of an insecticidal toxin produced by Enterobacter sp. strain 532 isolated from diseased Bombyx mori silkworms
FEMS Microbiol. Lett. 1;366(2).
http://doi.org/10.1093/femsle/fny295


2018年 9本

Masuda A., Minamihata K., Hino M., Morokuma D,. Karasaki N., Mon H., Kamiya N., Kusakabe T., Lee JM. (2018)
Modular surface display for porcine circovirus type 2 virus-like particles mediated by microbial transglutaminase
J. Insect. Biotechnol. and Sericol. 87.2. p. 2_053-2_060
https://doi.org/10.11416/jibs.87.2_053

Shiroishi M., Ito Y., Shimokawa K., Lee JM., Kusakabe T., Ueda T. (2018)
Structure-function analyses of a stereotypic rheumatoid factor unravel the structural basis for germline-encoded antibody autoreactivity
J. Biol. Chem. 4;293(18):7008-7016.
http://www.jbc.org/content/293/18/7008.full

Xu J.†, Morio A.†, Morokuma D., Nagata Y., Hino M., Masuda A., Li Z., Mon H., Kusakabe T., Lee JM. (2018)
†Both authors contributed equally to this manuscript
A functional polypeptide N-acetylgalactosaminyltransferase (PGANT) initiates O-glycosylation in cultured silkworm BmN4 cells
Appl. Microbiol. Biotechnol. 102(20):8783-8797.
https://doi.org/10.1007/s00253-018-9309-6

Morifuji Y., Xu J., Karasaki N., Iiyama K., Morokuma D., Hino M., Masuda A., Yano T., Mon H., Kusakabe T., Lee JM. (2018)
Expression, purification, and characterization of recombinant human α1-antitrypsin produced using silkworm-baculovirus expression system
Mol. Biotechnol. 60(12):924-934.
https://doi.org/10.1007/s12033-018-0127-y

Masuda A., Lee JM., Miyata T., Sato T., Hayashi S., Hino M., Morokuma D., Karasaki N., Mon H., Kusakabe T. (2018)
Purification and characterization of immunogenic recombinant virus-like particles of porcine circovirus type 2 expressed in silkworm pupae
J. Gen. Virol. 99:917-926
https://doi.org/10.1099/jgv.0.001087

Xxxx P., Minamihata K., Tatsuke T., Lee JM., Kusakabe T., Kamiya N. (2018)
Expression and activation of horseradish peroxidase-protein A/G fusion protein in silkworm larvae for diagnostic purposes
J. Biotechnol. 13,6,e1700624.
https://doi.org/10.1002/biot.201700624

Morokuma D., Hino M., Tsuchioka M., Masuda A., Mon H., Fujiyama K., Kajiura H., Kusakabe T., Lee JM. (2018)
A truncated form of human alpha 1-acid glycoprotein is useful as a molecular tool for insect glycobiology
Int. J. Indust. Entomol. 36(1) 15-24 (2018)
http://dx.doi.org/10.7852/ijie.2018.36.1.15

Masuda A., Xu J., Minamihata K., Kagawa G., Hamada Y., Morifuji Y., Yano T., Hino M., Morokuma D., Karasaki N., Mon H., Kamiya N., Kusakabe T., Lee JM. (2018)
Production of a biologically active human basic fibroblast growth factor using silkworm-baculovirus expression vector system
J. Asia-Pacific Entomol. 21,2,716-720.
https://doi.org/10.1016/j.aspen.2018.05.002

Jin S., Cheng T., Guo Y., Lin P., Zhao P., Liu C., Kusakabe T., Xia Q. (2018)
Bombyx mori epidermal growth factor receptor is required for nucleopolyhedrovirus replication
Insect Mol. Biol. 27,4,464-477
https://doi.org/10.1111/imb.12386


2017年 10本

Li Z., Cui Q., Xu J., Cheng D., Wang X., Li B., Lee JM., Xia Q., Kusakabe T., Zhao P. (2017)
SUMOylation regulates the localization and activity of Polo-like kinase 1 during cell cycle in the silkworm, Bombyx mori
Sci Rep. 14,7,1,15536.
https://www.nature.com/articles/s41598-017-15884-7

Patil AA., Tatsuke T., Mon H., Lee JM., Morokuma D., Hino M., Kusakabe T. (2017)
Molecular characterization of mitochondrial Zucchini and its relation to nuage-piRNA pathway components in Bombyx mori ovary-derived BmN4 cells
Biochem. Biophys. Res. Commun. 18,493,2,971-978.
https://doi.org/10.1016/j.bbrc.2017.09.107

Ji MM., Lee JM., Mon H., Iiyama K., Tatsuke T., Morokuma D., Hino M., Yamashita M., Hirata K., Kusakabe T. (2017)
Lipidation of BmAtg8 is required for autophagic degradation of p62 bodies containing ubiquitinated proteins in the silkworm, Bombyx mori
Insect Biochem. Mol. Biol. 89,86-96.
https://doi.org/10.1016/j.ibmb.2017.08.006

Patil AA., Tatsuke T., Mon H., Lee JM., Morokuma D., Hino M., Kusakabe T. (2017)
Characterization of Armitage and Yb containing granules and their relationship to nuage in ovary-derived cultured silkworm cell
Biochem. Biophys. Res. Commun. 19,490,2,134-140.
https://doi.org/10.1016/j.bbrc.2017.06.008

Iiyama K., Takahashi E., Lee JM., Mon H., Morishita M., Kusakabe T., Yasunaga-Aoki C., (2017)
Alkaline protease contributes to pyocyanin production in Pseudomonas aeruginosa
FEMS Microbiol. Lett.
https://doi.org/10.1093/femsle/fnx051

Yamashita M., Xu J., Morokuma D., Hirata K., Hino M., Mon H., Takahashi M., Hamdan SM., Sakashita K., Iiyama K., Banno Y., Kusakabe T., Lee JM. (2017)
Characterization of recombinant Thermococcus kodakaraensis (KOD) DNA polymerases produced using silkworm-baculovirus expression vector system
Mol. Biotechnol. 59,6,221-233.
https://doi.org/10.1007/s12033-017-0008-9

Mon H., Lee JM., Sato M., Kusakabe T. (2017)
Identification and functional analysis of outer kinetochore genes in the holocentric insect Bombyx mori
Insect Biochem. Mol. Biol. 86,1-8.
https://doi.org/10.1016/j.ibmb.2017.04.005

Morokuma D., Xu J., Hino M., Mon H., Merzaban JS., Takahashi M., Kusakabe T., Lee JM. (2017)
Expression and characterization of human β-1, 4-Galactosyltransferase 1 (β4GalT1) using silkworm-baculovirus expression system
Mol. Biotechnol. 59,4-5,151-158.
https://doi.org/10.1007/s12033-017-0003-1

Iiyama K., Morishita M., Lee JM., Mon H., Kusakabe T., Tashiro K., Akasaka T., Yasunaga-Aoki C., Miyamoto K. (2017)
A reconsideration of the taxonomic position of two bacterial strains isolated from flacherie-diseased silkworms in 1965
J. Insect Biotechnol. and Sericol. 86.2.p. 2_035-2_041
https://doi.org/10.11416/jibs.86.2_035S

Satone H., Nonaka S., Lee JM., Shimasaki Y., Kusakabe T., Kawabata S., Oshima Y. (2017)
Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripe
Toxicon 125.50-52
https://doi.org/10.1016/j.toxicon.2016.11.245


2016年 10本

Hasan MN., Hosen MJ., Thakur PK., Abir RA., Zubaer A., Renkai G., Yoshida M., Ohta H., Lee JM., Kusakabe T., Hirashima A. (2016)
In vitro screening for inhibitor of cloned Drosophila melanogaster tyramine-β-hydroxylase and docking studies
Int. J. Biol. Macromol. 93, Pt A, 889-895.
https://doi.org/10.1016/j.ijbiomac.2016.06.026

Hino M., Morokuma D., Mon H., Lee JM., Kusakabe T. (2016)
Characterization of the roles of DNA polymerases, clamp, and clap loaders during S-phase progression and cell cycle regulation in the silkworm, Bombyx mori
J. Insect Biotechnol. and Sericol. 85,2 p. 2_021-2_029
https://doi.org/10.11416/jibs.85.2_021

Ji MM., Lee JM., Mon H., Xu J., Tatsuke T., Kusakabe T. (2016)
Proteasome inhibitor MG132 impairs autophagic flux through compromising formation of autophagosomes in Bombyx cells
Biochem. Biophys. Res. Commun. 28,479,4,690-696.
https://doi.org/10.1016/j.bbrc.2016.09.151

Iiyama K.†, Lee JM.†, Tatsuke T., Mon H., Kusakabe T. (2016)
†Both authors contributed equally to this manuscript
Expression and characterization of recombinant Serratia liquefaciens nucleases produced with baculovirus-mediated silkworm expression system
Mol. Biotechnol. 58,6,393-403.
https://doi.org/10.1007/s12033-016-9937-y

Hino M., Kawanami T., Xu J., Morokuma D., Hirata K., Yamashita M., Karasaki N., Tatsuke T., Mon H., Iiyama K., Kamiya N., Banno Y., Kusakabe T., Lee JM. (2016)
High-level expression and purification of biologically active human IL-2
using silkworm-baculovirus expression vector system
J. Asia-Pacific. Entomol. 19,313–317
https://doi.org/10.1016/j.aspen.2016.03.014

Choi Ji-Hyun., Kim Dae-Jung., Sun Mee Hong., Sun-Jung Jo., Kwan-Sik Min., Young Chang Sohn., Lee JM., Kusakabe T. (2016)
Molecular analysis and bioactivity of luteinizing hormone from Japanese eel, Anguilla japonica, produced in silkworm pupae
Biotechnol. and Bioprocess Engineering. 21,3, pp 381–388
https://doi.org/10.1007/s12257-016-0042-7

Chen Jianping., Xu J., Hino M., Yamashita M., Hirata K., Anandrao Ashok Patil., Tatsuke T., Mon H., Banno Y,. Kusakabe T., Lee JM. (2016)
Co-expression of silkworm allatostatin-C receptor BNGR-A1 with its cognate G protein subunits enhances the GPCR display on the budding baculovirus
J. Asia-Pacific. Entomol. 19,753–760
https://doi.org/10.1016/j.aspen.2016.07.007

Takahashi E., LeeJM., Mon H., Chieda Y., Yasunaga-Aoki C., Kusakabe T., Iiyama K. (2016)
Effect of antibiotics on extracellular protein level in Pseudomonas aeruginosa 
Plasmid 84–85 (2016) 44–50
http://dx.doi.org/10.1016/j.plasmid.2016.03.001

Taira E., Mon H., Lee JM., Kusakabe T., C Yasunaga-Aoki., Iiyama K. (2016)
Virulence of lipopolysaccharide-deficient mutants of Serratia liquefaciens toward the silkworm, Bombyx mori
J. Insect Biotechnol. and Sericol. 85,1 p. 1_007-1_014
https://doi.org/10.11416/jibs.85.1_007

Hamajima R., Yasunaga-Aoki C., Iwanaga M., Imanishi S., Kobayashi S., Sasaki K., Kusakabe T., Lee JM., Mon H., Kobayashi M., Ikeda M. (2016)
rRNA degradation in Bombyx mori and Bombyx mandarina cells infected with heterologous nucleopolyhedroviruses
J. Insect Biotechnol. and Sericol. 85,3 p. 3_073-3_077
https://doi.org/10.11416/jibs.85.3_073


2015年 15本

Zhu L., Mon H., Xu J., Lee JM., Kusakabe T. (2015)
CRISPR/Cas9-mediated knockout of factors in non-homologous end joining pathway enhances gene targeting in silkworm cells
Sci. Rep. 10,5,18103.
https://doi.org/10.1038/srep18103

Xu J., Zhang P., Kusakabe T., Mon H., Li Z., Zhu L., Iiyama K., Banno Y., Morokuma D., Lee JM. (2015)
Comparative proteomic analysis of hemolymph proteins from Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-sensitive or -resistant silkworm strains during infections
Comp. Biochem. Physiol. Part D Genomics Proteomics 16,36-47.
https://doi.org/10.1016/j.cbd.2015.07.003

Imai S., Kusakabe T., Xu J., Li Z., Shirai S., Mon H., Morokuma D., Lee JM. (2015)
Roles of silkworm endoplasmic reticulum chaperones in the secretion of recombinant proteins expressed by baculovirus system
Mol. Cell. Biochem. 409,1,255-262.
https://doi.org/10.1007/s11010-015-2529-5

Zhu L., Tatsuke T., Xu J., Li Z., Mon H., Lee JM., Kusakabe T. (2015)
Loqs depends on R2D2 to localize in D2 body-like granules and functions in RNAi pathways in silkworm cells
Insect Biochem. Mol. Biol. 64,78-90.
https://doi.org/10.1016/j.ibmb.2015.07.011

Hayashi K., Lee JM., Tomozoe Y., Kusakabe T., Kamiya N. (2015)
Heme precursor injection is effective for Arthromyces ramosus peroxidase fusion protein production by a silkworm expression system
J Biosci. Bioeng. 120,4,384-6.
https://doi.org/10.1016/j.jbiosc.2015.02.013

Hashiguchi Y., Lee JM., Shiraishi M., Komatsu S., Miki S., Shimasaki Y., Mochioka N., Kusakabe T., Oshima Y. (2015)
Characterization and evolutionary analysis of tributyltin-binding protein and pufferfish saxitoxin and tetrodotoxin-binding protein genes in toxic and nontoxic pufferfishes
J. Evol. Biol. 28,5,1103-18.
https://doi.org/10.1111/jeb.12634

Masuda A., Xu J., Mitsudome T., Nagata Y., Morokuma D., Mon H., Banno Y., Kusakabe T., Lee JM. (2015)
Mass production of an active peptide-N-Glycosidase F using silkworm-baculovirus expression system
Mol. Biotechnol. 57,8,735-45.
https://doi.org/10.1007/s12033-015-9866-1

Sugahara R., Jouraku A., Nakakura T., Kusakabe T., Yamamoto T., Shinohara Y., Miyoshi H., Shiotsuki T. (2015)
Two adenine nucleotide translocase paralogues involved in cell proliferation and spermatogenesis in the silkworm Bombyx mori
PLoS One. 5,10,3,e0119429.
https://doi.org/10.1371/journal.pone.0119429

Mitsudome T., Mon H., Xu J., Li Z., Lee JM., Patil AA., Masuda A., Iiyama K., Morokuma D., Kusakabe T. (2015)
Biochemical characterization of maintenance DNA methyltransferase DNMT-1 from silkworm, Bombyx mori
Insect Biochem. Mol. Biol. 58,55-65.
https://doi.org/10.1016/j.ibmb.2015.01.008

Masuda A., Xu J., Mitsudome T., Morokuma D., Mon H., Banno Y., Kusakabe T., Lee JM. (2015)
Improvement of Endo-β-N-acetylglucosaminidase H production using
silkworm–baculovirus protein expression system.
J. Asia-Pacific. Entomol. 18,175–180
https://doi.org/10.1016/j.aspen.2015.01.006

Morokuma D., Xu J., Mon H., Hirata K., Hino M., Kuboe S., Yamashita M., Kusakabe T., Lee JM. (2015)
Human alpha 1-acid glycoprotein as a model protein for glycoanalysis in baculovirus expression vector system
J. Asia-Pacific. Entomol. 18,303–309
https://doi.org/10.1016/j.aspen.2015.03.006

Sun Mee Hong, Ji-Hyun Choi, Sun-Jung Jo, Seong Kyu Song, Lee JM., Kusakabe T. (2015)
Expression of recombinant viscum album coloratum lectin B-chain in the silkworm expression system and evaluation of antioxidant activity
Biotechnol. and Bioprocess Engineering. 20,3,515–522
https://doi.org/10.1007/s12257-014-0806-x

Morokuma D, Mon H., Banno Y., Kusakabe T., Lee JM. (2015)
Differential N-Glycan Modifications of Human Alpha 1-Acid Glycoprotein (α1AGP) Produced in Different Silkworm Strains using the Baculovirus Expression System
J. Insect Biotechnol. and Sericol. 84(2):49-53
https://doi.org/10.11416/jibs.84.2_04

Iiyama K., Mon H., Mori K., Mitsudome T., Lee JM., Kusakabe T., Tashiro K., Asano S., Yasunaga-Aoki C. (2015)
Characterization of KfrA proteins encoded by a plasmid of Paenibacillus popilliae ATCC 14706T
Meta Gene 4, 29-44
https://doi.org/10.1016/j.mgene.2015.03.001

Kamada Y., Kusakabe T., Sugimoto Y. (2015)
Amylodgenic lysozymes accumulate in the endoplasmic reticulum accompanied by the augmentation of ER stress signals
Biochimica et Biophysica Acta (BBA) – General Subjects
https://doi.org/10.1016/j.bbagen.2015.01.018

国際学会での発表(2015年以降)

2020年


2019年

Hino M., Mon H., Fujii T., Lee JM, Banno Y., Kusakabe T.
An attempt to construct silkworm artificial chromosome
Eighth International Symposium on Molecular Insect Science.

Kakino K., Mon H., Sato M., Fujita R.,Lee JM., Kusakabe T.
A testis-specific uncharacterized gene unique to silkworm is necessary for cell progression and survival
International Congress on Sericulture and Silk Industry.


2018年

Kobayashi M., Mon H., Lee JM., Kusakabe T.
Construction of Exon Trap Vectors for Isolating the Reporter Cell Lines from Silkworm Cultured Cells
International Symposium on Agricultural, Food, Environmental and Life Science in Asia.

Hino M., Mon H., Lee JM., Kusakabe T.
Bombyx mori Orc2 interacts with HP1 via PXVXL motif
International Symposium on Agricultural, Food, Environmental and Life Science in Asia.


2017年

Kinoshita Y., Masuda A., Morio A., Karasaki N., Tatsuke T., Mon H., Lee JM., Kusakabe T.
High yield expression and purification of Bovine aprotinin by using the silkworm-baculovirus expression vector system
International Symposium on Agriculture, Food, Environmental and Life Science in Asia.

Kinoshita Y., Masuda A., Morio A., Karasaki N., Tatsuke T., Mon H., Lee JM., Kusakabe T.
Characterization of proprotein convertase-furin expressed using the silkworm-baculovirus expression system
Asia-Pacific Congress of Sericulture and Insect Biotechnology.

Masuda A.,

International Symposium on Agriculture, Food, Environmental and Life Science in Asia.

Masuda A.,

Asia-Pacific Congress of Sericulture and Insect Biotechnology.

Morifuji Y., Karasaki N, Iiyama K., Morokuma D., Lee JM., and Kusakabe T.
Expression, Purification and Characterization of Recombinant α1-antitrypsin Produced by Silkworm-Baculovirus Expression System
International Symposium on Agriculture, Food, Environmental and Life Science in Asia.

Morifuji Y., Masuda A., Yano T., Mon H., Lee JM., Kusakabe T.
Analyses of the roles of silkworm insulator proteins BmCTCF and BmCP190 on enhancer-promoter interaction
Asia-Pacific Congress of Sericulture and Insect Biotechnology.

Morio A., Kinoshita Y., Masuda A., Karasaki N., Morokuma D., Lee JM., Kusakabe T.
Exploring High-Efficient Purification Method for Endo-a-N-acetylgalactosaminidase from Enterococcus faecalis ( EndoEF ) Expressed by Baculovirus Expression System
International Symposium on Agriculture, Food, Environmental and Life Science in Asia.

Morio A., Jian Xu, Kinoshita Y., Masuda A., Karasaki N., Morokuma D., Mon H., Lee JM., Kusakabe T.
Human and silkworm glycoproteins as reporter protein for O-glycosylation analysis in insect cells
Asia-Pacific Congress of Sericulture and Insect Biotechnology.

Yano T., Hino M, Tatsuke T., Mon H., Lee JM., Kusakabe T.
Comparative analysis of linker histone H1 variants in Bombyx mori
International Symposium on Agriculture, Food, Environmental and Life Science in Asia.

Yano T., Morifuji Y., Hino M., Tatsuke T., Mon H., Lee JM., Kusakabe T.
Characterization of histone H1 variants in Bombyx mori
Asia-Pacific Congress of Sericulture and Insect Biotechnology.


2016年

Tsuchioka M., Mon H., Lee JM., Tatsuke T., Kusakabe T.
Functional Analysis of histone acetylation in Bombyx mori
Asia-Pacific Congress of Sericulture and Insect Biotechnology.

Morokuma D., Mon H., Lee JM., Kusakabe T.
Synthesis of complex-type N-glycans on recombinant proteins produced by silkworm-baculovirus expression system
International Congress of Entomology.


2015年

Chen Jianping, Jian Xu, Kusakabe T.
Engineering of silkworm-baculovirus expression system for efficient production of G protein-coupled receptors
Asia-Pacific Congress of Sericulture and Insect Biotechnology.

Ji MM., Lee JM., Mon H., Zhiqing Li, Jian Xu, Li Zhu, Kuboe S., Imai S., Chen Jianping, Mitsudome T., Masuda A., Morokuma D., Hino M, Yamashita M., Hirata K., Kusakabe T.
GFP-p62 degradation is an excellent marker of autophagic flux in Bombyx
Asia-Pacific Congress of Sericulture and Insect Biotechnology.

Morokuma D., Jian Xu, Mon H., Lee JM., Kusakabe T.
Synthesis of Complex-Type N-Glycans on Recombinant Proteins by Modifying N-Glycosylation Pathway in Cultured Silkworm Cells
Asia-Pacific Congress of Sericulture and Insect Biotechnology.

Hino M., Mon H., Li Zhu1, Yamashita M., Hirata K., Dan Zheng, Jian Xu, Ji MM., Morokuma D.,Lee JM, Kusakabe T.
Characterization of silkworm replication related proteins for making an artificial chromosome
Asia-Pacific Congress of Sericulture and Insect Biotechnology.

Hirata K., Jian Xu, Hino M., Yamashita M., Mon H., Lee JM., Kusakabe T.
Construction The System of Establishing New Silkworm Cell Lines by Using Cell Fusion
Asia-Pacific Congress of Sericulture and Insect Biotechnology.

Yamashita M., Hino M., Hirata K., Jian Xu, Ji MM., Morokuma D., Mon H., Lee JM., Kusakabe T.
Characterization of BmNPV promoters for improving exogenous genes expression in Bombyx cells
Asia-Pacific Congress of Sericulture and Insect Biotechnology.