研 究 成 果

論文発表

[2021]

1) Matsunaga E, Tanaka Y, Toyota S, Yamada H, Oka T, Higuchi Y, Takegawa K:

Identification and characterization of β-D-galactofuranosidases from Aspergillus nidulans and Aspergillus fumigatus.

J. Biosci. Bioeng. 131, 1-7 (2021)


2) Kawano-Kawada M, Ueda T, Mori H, Ichimura H, Takegawa K, Sekito T:

Stm1 is a vacuolar PQ-loop protein involved in the transport of basic amino acids in Schizosaccharomyces pombe.

Biochim. Biophys. Acta Biomembr. 1863, 183507 (2021)


3) Tanaka N, Kagami A, Hirai K, Suzuki S, Matsuura S, Fukunaga T, Tabuchi M, Takegawa K:

The fission yeast gmn2+ gene encodes an ERD1 homologue of Saccharomyces cerevisiae required for protein glycosylation and retention of luminal endoplasmic reticulum proteins.

J. Gen. Appl. Microbiol. 67, 67-76 (2021)


4) Higuchi Y:

Membrane traffic related to endosome dynamics and protein secretion in filamentous fungi.

Biosci. Biotechnol. Biochem. 85, 1038-1045 (2021)


5) Klionsky DJ et al.:

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition).

Autophagy 17, 1-382 (2021)


6) Fukunaga T, Tanaka N, Furumoto T, Nakakita S, Ohashi T, Higuchi Y, Maekawa H, Takegawa K:

Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.

Glycobiology 31, 1037-1045 (2021)


7) Higuchi Y:

Membrane traffic in Aspergillus oryzae and related filamentous fungi.

J. Fungi 7, 534 (2021)


8) Morita Y, Katakura Y, Takegawa K, Higuchi Y:

Correlative localization analysis between mRNA and EGFP-fused protein by single-molecule FISH using an egfp probe in Aspergillus oryzae.

Front. Fungal Biol. in press




[2020]

1) Li Q, Higuchi Y, Tanabe K, Katakura Y, Takegawa K:

Secretory production of N-glycan-deleted glycoprotein in Aspergillus oryzae.

J. Biosci. Bioeng. 129, 573-580 (2020)


2) Chihara Y, Tanaka Y, Izumi M, Hagiwara D, Watanabe A, Takegawa K, Kamei K, Shibata N, Ohta K, Oka T:

Biosynthesis of β-(1→5)-galactofuranosyl chains of fungal-type and O-mannose-type galactomannans within the invasive pathogen Aspergillus fumigatus.

mSphere 5, e00770-19 (2020)


3) Iwaki Y, Matsunaga E, Takegawa K, Sato C, Kitajima K:

Identification and characterization of a novel, versatile sialidase from a Sphingobacterium that can hydrolyze the glycosides of any sialic acid species at neutral pH.

Biochem. Biophys. Res. Commun. 523, 487-492 (2020)


4) Ashida H, Fujimoto T, Kurihara S, Nakamura M, Komeno M, Huang Y, Katayama T, Kinoshita T, Takegawa K:

1,6-alpha-Fucosidases from Bifidobacterium longum subsp. Infantis ATCC15697 involved in the degradation of core-fucosylated N-glycan.

J. Appl. Glycosci. 67, 23-29 (2020)


5) Shen L, Viljoen A, Villaume S, Maju J, Chene L, Mery A, Fabre E, Takegawa K, Lowary TL, Vincent SP, Kremer L, Guerardel Y, Mariller C:

GlfH1, an endogenous remodeling galactofuranosidase that hydrolyzes mycobacterial arabinogalactan.

J. Biol. Chem. 295, 5110-5123 (2020)


6) Maekawa H, Takegawa K:

Yeast flocculin: methods for quantitative analysis of flocculation in yeast cells.

Methods Mol. Biol. 2132, 437-444 (2020)


7) Fukunaga T, Tanaka N, Furumoto T, Nakakita S, Ohashi T, Higuchi Y, Maekawa H, Takegawa K:

Characterization of N- and O-linked galactosylated oligosaccharides from fission yeast species.

J. Biosci. Bioeng. 130, 128-136 (2020)


8) 竹川 薫、樋口 裕次郎:

エンドグリコシダーゼを用いたバイオ医薬品糖タンパク質の糖鎖改変技術

バイオサイエンスとインダストリー 78, 217-220 (2020)


9) 阪井 康能、竹川 薫、橋本 渉、片山 高嶺:

遺伝子・細胞から見た応用微生物学

朝倉書店 ISBN: 978-4-254-43124-7 (2020)


10) Ohashi T, Tanaka T, Tanaka N, Takegawa K:

SpMnn9p and SpAnp1p form a protein complex involved in mannan synthesis in the fission yeast Schizosaccharomyces pombe.

J. Biosci. Bioeng. 130, 335-340 (2020)


11) Papali Tautau FA, Izumi M, Matsunaga E, Higuchi Y, Takegawa K:

Microbial α-L-rhamnosidases of glycosyl hydrolase families GH78 and GH106 have broad substrate specificities toward α-L-rhamnosyl- and α-L-mannosyl-linkages.

J. Appl. Glycosci. 67, 87-93 (2020)


12) Morita Y, Kikumatsu F, Higuchi Y, Katakura Y, Takegawa K:

Characterization and functional analysis of ERAD-related AAA+ ATPase Cdc48 in Aspergillus oryzae.

Fungal Biol. 124, 801-813 (2020)


13) Ohashi T, Hegi S, Fukunaga T, Hosomi A, Takegawa K:

Golgi localization of glycosyltransferases requires Gpp74p in Schizosaccharomyces pombe.

Appl. Microbiol. Biotechnol. 104, 8897-8909 (2020)


14) Higuchi Y, Takegawa K:

Single-molecule FISH reveals subcellular localization of α-amylase and actin mRNAs in the filamentous fungus Aspergillus oryzae.

Front. Microbiol. 11, 578862 (2020)


15) Takegawa K, Oka T:

Glycan-mediated interactions between fungal and higher animal cells. Health and Disease in Glycomedicine.

Comprehensive Glycoscience 2nd Edition. Barchi Jr. JJ eds, Elsevier (2020)




[2019]

1) 梶原 康博、大石 雅志、竹川 薫、高下 秀春:

微量香気成分インドールが大麦焼酎の官能特性に及ぼす影響

日本醸造協会誌 114, 1, 53-56 (2019)


2) Ota R, Okamoto Y, Vavricha CJ, Oka T, Matasunaga E, Takegawa K, Kiyota H, Izumi M:

Chemo-enzymatic synthesis of p-nitrophenyl β-D-galactofuranosyl disaccharides from Aspergillus sp. fungal-type galactomannan.

Carbohydr. Res. 473, 99-103 (2019)


3) Tominaga A, Higuchi Y, Mori H, Akai M, Suyama A, Yamada N, Takegawa K:

Catechol O-methyltransferase homologs in Schizosaccharomyces pombe are response factors to alkaline and salt stress.

Appl. Microbiol. Biotechnol. 103, 4881-4887 (2019)


4) Senicar M, Legentil L, Ferrieres V, Eliseeva SV, Petoud S, Takegawa K, Lafite P, Daniellou R:

Galactofuranosidase from JHA19 Streptomyces sp.: subcloning and biochemical characterization.

Carbohydr. Res. 480, 35-41 (2019)


5) Takegawa K:

14.6 Carbohydrate-Mediated Interactions. Between Microbial and Higher Animal Cells (Fungi). Glycoscience: Basic Science to Application. Inshights from the Japan Consortium for Glycobiology and Glycotechnology (JCGG). Taniguchi N, Endo T, Hirabayashi J, Nishihara S, Kadomatsu K, Akiyoshi K, Aoki-Kinoshita KF eds.

Springer (2019)


6) Seki H, Huang Y, Arakawa T, Yamada C, Kinoshita T, Iwamoto S, Higuchi Y, Takegawa K, Fushinobu S:

Structural basis for the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris.

J. Biol. Chem. 294, 17143-17154 (2019)




[2018]

1) Higuchi Y, Mori H, Kubota T, Takegawa K:

Analysis of ambient pH stress response mediated by iron and copper intake in Schizosaccharomyces pombe.

J. Biosci. Bioeng. 125, 92-96 (2018)


2) Kamatani S, Takegawa K, Kimura Y:

Catalytic activity profile of polyphosphate kinase 1 from Myxococcus xanthus.

Curr. Microbiol. 75, 379-385 (2018)


3) Huang Y, Higuchi Y, Kinoshita T, Mitani A, Eshima Y, Takegawa K:

Characterization of novel endo-β-N-acetylglucosaminidases from Sphingobacterium species, Beauveria bassiana and Cordyceps militaris that specifically hydrolyze fucose-containing oligosaccharides and human IgG.

Sci. Rep. 8, 246 (2018)


4) 樋口 裕次郎:

ダイナミックなオルガネラ:初期エンドソーム

生物工学会誌 96, 79 (2018)


5) Kimura Y, Yamamoto Y, Kajimoto S, Sakai A, Takegawa K:

Substrate specificity of Nudix hydrolases from Myxococcus xanthus.

J. Gen. Appl. Microbiol. 64, 94-98 (2018)


6) Sugiura A, Nguyen LC, Shamim HM, Iida N, Nakamura Y, Iida T, Nakase M, Takegawa K, Senda M, Jida S, Ueno M:

Mutation in fission yeast phosphatidylinositol 4-kinase Pik1 is synthetically lethal with defect in telomere protection protein Pot1.

Biochem. Biophys. Res. Commun. 496, 1284-1290 (2018)


7) Huang Y, Higuchi Y, Mori K, Yamashita R, Okino N, Tashiro K, Takegawa K:

Draft genome sequence of Sphingobacterium sp. HMA12, which encodes endo-β-N-acetylglucosaminidases and can specifically hydrolyze fucose-containing oligosaccharides.

Genome Announc. 6, e01525-17 (2018)


8) Kajiwara Y, Mori K, Tashiro K, Higuchi Y, Takegawa K, Takashita H:

Genomic sequence of Saccharomyces cerevisiae BAW-6, a yeast strain optimal for brewing barley shochu.

Genome Announc. 6, e00228-18 (2018)


9) 樋口 裕次郎:

糸状菌における初期エンドソーム動態の生理的意義

バイオサイエンスとインダストリー 76, 218-221 (2018)


10) Higuchi Y, Matsufuji H, Tanuma M, Arakawa T, Mori K, Yamada C, Shofia R, Matsunaga E, Tashiro K, Fushinobu S, Takegawa K:

Identification and characterization of a novel β-D-galactosidase that releases pyruvylated galactose.

Sci. Rep. 8, 12013 (2018)


11) Higuchi Y, Matsufuji H, Mori K, Matsunaga E, Tashiro K, Takegawa K:

Draft genome sequence of Bacillus sp. HMA207, a strain that exhibits β-D-galactosidase activity to release pyruvylated galactose.

Microbiol. Resour. Announc. 7, e01169-18 (2018)


12) 竹川 薫:

農芸化学らしさを世界に伝える

化学と生物 56, 451-452 (2018)




[2017]

1) Higuchi Y, Eshima Y, Huang Y, Kinoshita T, Sumiyoshi W, Nakakita S, Takegawa K:

Highly efficient transglycosylation of sialo-complex-type oligosaccharide using Coprinopsis cinerea endoglycosidase and sugar oxazoline.

Biotechnol. Lett. 39, 157-162 (2017)


2) Matsunaga E, Higuchi Y, Mori K, Yairo N, Toyota S, Oka T, Tashiro K, Takegawa K:

Characterization of a PA14 domain-containing galactofuranose-specific β-D-galactofuranosidase from Streptomyces sp.

Biosci. Biotechnol. Biochem. 81, 1314-1319 (2017)


3) Matsunaga E, Higuchi Y, Mori K, Tashiro K, Takegawa K:

Draft genome sequence of Streptomyces sp. JHA26, a strain that harbors a PA14 domain-containing β-D-galactofuranosidase.

Genome Announc. 5, e00190-17 (2017)


4) Katafuchi Y, Li Q, Tanaka Y, Shinozuka S, Kawamitsu Y, Izumi M, Ekino K, Mizuki K, Takegawa K, Shibata N, Goto M, Nomura Y, Ohta K, Oka T:

GfsA is a β1,5-galactofuranosyltransferase involved in the biosynthesis of the galactofuran side chain of fungal-type galactomannan in Aspergillus fumigatus.

Glycobiology 27, 568-581 (2017)


5) Suyama A, Higuchi Y, Urushihara M, Maeda Y, Takegawa K:

Production of 3-hydroxypropionic acid via the malonyl-CoA pathway using recombinant fission yeast strains.

J. Biosci. Bioeng. 124, 392-399 (2017)


6) Kwon HS, Kawaguchi K, Kikuma T, Takegawa K, Kitamoto K, Higuchi Y:

Analysis of an acyl-CoA binding protein in Aspergillus oryzae that undergoes unconventional secretion.

Biochem. Biophys. Res. Commun. 493, 481-486 (2017)


7) Maekawa H, Neuner A, Ruthnick D, Schiebel E, Pereira G, Kaneko Y:

Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeast Ogataea polymorpha.

eLife 6, e24340 (2017)


8) Tsukamura W, Kurogoshi M, Mori M, Osumi K, Matsuda A, Takegawa K, Furukawa K, Shirai T:

Preparation and biological activities of anti-HER2 monoclonal antibodies with fully core-fucosylated homogeneous bi-antennary complex-type glycans.

Biosci. Biotechnol. Biochem. 81, 2353-2359 (2017)


9) 竹川 薫、樋口 裕次郎:

ピルビン酸含有酸性糖鎖の生物における分布と役割

化学と生物 55, 738-742 (2017)


10) Togo Y, Higuchi Y, Katakura Y, Takegawa K:

Early endosome motility mediates α-amylase production and cell differentiation in Aspergillus oryzae.

Sci. Rep. 7, 15757 (2017)


11) Yamamoto K, Tran T, Takegawa K, Kaneko Y, Maekawa H:

Regulation of the mating type switching by the mating type genes and RME1 in Ogataea polymorpha.

Sci. Rep. 7, 16318 (2017)




[2016]

1) Higuchi Y, Mori K, Suyama A, Huang Y, Tashiro K, Kuhara S, Takegawa K:

Draft genome sequence of Bacillus clausii AKU0647, a strain that prduces endo-β-N-acetylglucosaminidase A.

Genome Announc. 4, e00310-16 (2016)


2) Higuchi Y, Yoshinaga S, Yoritsune K, Tateno H, Hirabayashi J, Nakakita S, Kanekiyo M, Kakuta Y, Takegawa K:

A rationally engineered yeast pyruvyltransferase Pvg1p introduces sialylation-like properties in neo-human-type complex oligosaccharide.

Sci. Rep. 6, 26349 (2016)


3) Oka M, Takegawa K, Kimura Y:

Lysyl-tRNA synthetase from Myxococcus xanthus catalyzes the formation of diadenosine penta- and hexaphosphates from adenosine tetraphosphate.

Arch. Biochem. Biophys. 604, 152-158 (2016)


4) Kawano-Kawada M, Chardwiriyapreecha S, Manabe K, Sekito T, Akiyama K, Takegawa K, Kakinuma Y:

The amino-terminal hydrophilic region of the vacuolar transporter Avt3p is dispensable for the vacuolar amino acid compartmentalization of Schizosaccahromyces pombe.

Biosci. Biotechnol. Biochem. 80, 2291-2297 (2016)


5) Schuster M, Martin-Urdiroz M, Higuchi Y, Hacker C, Kilaru S, Gurr S, Steinberg G:

Co-delivery of cell wall-forming enzymes in the same vesicle for coordinated fungal cell wall formation.

Nat. Microbiol. 1, 16149 (2016)


6) Kawaguchi K, Kikuma T, Higuchi Y, Takegawa K, Kitamoto K:

Subcellular localization of acyl-CoA binding protein in Aspergillus oryzae is regulated by autophagy machinery.

Biochem. Biophys. Res. Commun. 480, 8-12 (2016)




[2015]

1) Hosomi A, Higuchi Y, Yagi S, Takegawa K:

Vsl1p cooperates with Fsv1p for vacuolar protein transport and homotypic fusion in Schizosaccharomyces pombe.

Microbiology 161, 89-98 (2015)


2) Tsukamoto Y, Kagiwada S, Shimazu S, Takegawa K, Noguchi T, Miyamoto M:

Coordinated regulations by two VPS9 domain-containing guanine-nucleotide exchange factors.

Biochem. Biophys. Res. Commun. 458, 802-809 (2015)


3) Higuchi Y, Steinberg G:

Early endosomes motility in filamentous fungi: How and why they move.

Fungal Biol. Rev. 29, 1-6 (2015)


4) Higuchi Y:

Initial fungal effector production is mediated by early endosome motility.

Commun. Integr. Biol. 8, e1025187 (2015)


5) Kato T, Shirakawa Y, Takegawa K, Kimura Y:

Functional analysis of conserved motifs in a bacterial tyrosine kinase, BtkB, from Myxococcus xanthus.

J. Biochem. 158, 385-392 (2015)


6) Chardwiriyapreecha S, Manabe K, Iwaki T, Kawano-kawada M, Sekito T, Lnprom S, Akiyama K, Takegawa K, Kakinuma Y:

Functional expression and characterization of Schizosaccharomyces pombe Avt3p as a vacuolar amino acid exporter in Saccharomyces cerevisiae.

PLOS ONE 10, e0130542 (2015)


7) Oka M, Takegawa K, Kimura Y:

Enzymatic characterization of a class II lysyl-rRNA synthetase, LysS, from Myxococcus xanthus.

Arch. Biochem. Biophys. 579, 33-39 (2015)


8) Eshima Y, Higuchi Y, Kinoshita T, Nakakita S, Takegawa K:

Transglycosylation activity of glycosynthase mutants of endo-β-N-acetylglucosaminidase from Coprinopsis cinerea.

PLOS ONE 10, e0132859 (2015)


9) Matsunaga E, Higuchi Y, Mori K, Yairo N, Oka T, Shinozuka S, Tashiro K, Izumi M, Kuhara S, Takegawa K:

Identification and characterization of a galactofuranose-specific β-D-galactofuranosidase from Streptomyces species.

PLOS ONE 10, e0137230 (2015)


10) Matsunaga E, Higuchi Y, Mori K, Tashiro K, Kuhara S, Takegawa K:

Draft genome sequence of Streptomyces sp. JHA19, a strain that possesses β-D-galactofuranosidase activity.

Genome Announc. 3, e01171-15 (2015)




[2014]

1) Okamoto R, Takegawa K, Kimura Y:

Regulation of eukaryotic-like protein kinase activity of DspA from Myxococcus xanthus by autophosphotylation.

J. Biochem. 155, 99-106 (2014)


2) Futagami T, Seto K, Kajiwara Y, Takashita H, Omori T, Takegawa K and Goto M:

The putative stress sensor MtlA is required for conidia formation, cell wall stress tolerance, and cell wall integrity in Aspergillus nidulans.

Biosci. Biotechnol. Biochem. 78, 326-335 (2014)


3) Higuchi Y, Ashwin P, Roger Y, Steinberg G:

Early endosome motility spatially organizes polysome distribution.

J. Cell Biol. 204, 343-357 (2014)


4) Sasaki M, Takegawa K, Kimura Y:

Enzymatic characteristics of an ApaH-like phosphatase, PrpA, and a diadenosine tetraphosphate hydrolase, ApaH, from Myxococcus xanthus.

FEBS Lett. 588, 3395-3402 (2014)


5) Takegawa K, Matsuzawa T:

Insights into metabolism and the galactose recognition system from microarray analysis in the fission yeast Schizosaccharomyces pombe.

Microbial Production. From Genome Design to Cell Engineering. p109-118 Springer (2014)


6) Bielska E*, Higuchi Y*, Schuster M*, Steinberg N, Kilaru S, Talbot NJ, Steinberg G:

Long-distance endosome trafficking drives fungal effector production during plant infection.

Nat. Commun. 5, 5097 (2014) *These authors contributed equally.


7) Yoritsune K, Higuchi Y, Matsuzawa T, Takegawa K:

Functional analysis of putative phosphoenolpyruvate transporters localized to the Golgi apparatus in Schizosaccharomyces pombe.

FEMS Yeast Res. 14, 1101-1109 (2014)




[2013]

1) Matsuzawa T, Hara F, Tanaka N, Tohda H and Takegawa K:

ght2+ is required for UDP-galactose synthesis from extracellular galactose by Schizosaccharomyces pombe.

Appl. Microbiol. Biotechnol. 97, 4957-4964 (2013)


2) Matsuzawa T, Kageyama Y, Ooishi K, Kawamukai M, and Takegawa K:

The zinc finger protein Gsf1 regulates Gsf2-dependent flocculation in fission yeast.

FEMS Yeast Res. 13, 259-266 (2013)


3) Yoritune K, Matsuzawa T, Ohashi T, and Takegawa K:

The fission yeast Pvg1p shows galactose-specific pyruvyltransferase activity.

FEBS Lett. 587, 917-921 (2013)


4) Tashiro S, Futagami T, Wada S, Kajiwara Y, Takashita H, Omori T, Takahashi T, Yamada O, Takegawa K, and Goto M:

Development of genetic tools for white koji mold, Aspergillus kawachii.

J. Gen. Appl. Microbiol. 59, 257-260 (2013)


5) Matsuzawa T, Tohda H, and Takegawa K:

Ethanol-inducible gene expression using gld1+ promoter in the fission yeast Schizosaccharomyces pombe.

Appl. Microbiol. Biotechnol. 97, 6835-6843 (2013)


6) Sasaki M, Kumagai H, Takegawa K, and Tohda H:

Characterization of genome-reduced fission yeast strains.

Nucleic Acids Res. 41, 5382-5399 (2013)


7) Futagami T, Fukaki Y, Fujihara H, Takegawa K, Goto M, and Furukawa K:

Evaluation of the inhibitory effects of chloroform on ortho-chlorophenol- and chloroethene-dechlorinating Desulfitobacterium strains.

AMB Express 3, 30 (2013)


8) Nakase Y, Nakase M, Kashiwazaki J, Murai T, Otsubo Y, Mabuchi I, Yamamoto M, Takegawa K, and Matsumoto T:

Fission yeast Any1, a beta-arrestin-like protein, is involved in TSC-Rheb signaling and regulates amino acid transporters.

J. Cell Sci. 126, 3972-3981 (2013)


9) Pongharoen P, Kawano-Kawada M, Iwaki T, Sugimoto N, Sekito T, Akiyama K, Takegawa K, and Kakinuma Y:

Functional expression of Schizosaccharomyces pombe Vba2p in vacuolar membrane of Saccharomyces cerevisiae.

Biosci. Biotechnol. Biochem. 77, 1988-1990 (2013)


10) Komachi Y, Hatakeyama S, Motomatsu H, Futagami T, Kinzjakina K, Sorbado P, Ekino K, Takegawa K, Goto M, Nomura Y, and Oka T:

gfsA encodes a novel galactofuranosyltransferase involved in biosynthesis of galactofuranose antigen of O-glycan in Aspergillus nidulans and A. fumigatus.

Mol. Microbiol. 90, 1054-1073 (2013)


11)竹川 薫、松沢智彦:

6章 タンパク質の分泌、「酵母の生命科学と生物工学 ー産業応用から基礎科学へー」 DOJIN BIOSCIENCE シリーズ、p83-96、化学同人 (2013)




[2012]


1) Mukaiyama H, Kodera M, Tanaka N, and Takegawa K:

N-glycans are not required for the efficient degradation of the mutant Saccharomyces cerevisiae CPY* in Schizosaccharomyces pombe.

Appl. Microbiol. Biotechnol. 93, 1609-1618 (2012)


2) Matsuzawa T, Fujita Y, Tohda H, and Takegawa K:

Snf1-like protein kinase Ssp2 regulates glucose derepression in Schizosaccharomyces pombe.

Eukaryot. Cell 11, 159-167 (2012)


3) Matsuzawa T, Yoritsune K, and Takegawa K:

MADS box transcription factor Mbx2/Pvg4 regulates invasive growth and flocculation by inducing gsf2+ expression in fission yeast.

Eukaryot. Cell 11, 151-158 (2012)


4) Matsuzawa T, Mori K, Kadowaki T, Shimada M, Tashiro K, Kuhara S, Inagawa H, Soma G, and Takegawa K:

Genome sequence of the Pantoea agglomerans Strain IG1.

J. Bacteriol. 194, 1258-1259 (2012)


5) Nakase M, Nakase Y, Chardwiriyapreecha S, Kakinuma Y, Matsumoto T, and Takegawa K:

Intracellular trafficking and ubiquitination of the Schizosaccharomyces pombe amino acid permease Aat1p.

Microbiology 158, 659-673 (2012)


6) Kitamura K, Nakase M, Tohda H, and Takegawa K:

The ubiquitin ligase Ubr11 is essential for the oligopeptide utilization in fission yeast Schizosaccharomyces pombe.

Eukaryot. Cell 11, 302-310 (2012)


7) Nakase M, Tsukamoto Y, Hosomi A, Tsuji H, Matsuda T, Miyamoto M, and Takgawa K:

CUE domain-containing protein Vps901 is required for vacuolar protein transport in Schizosaccharomyces pombe.

Biosci. Biotechnol. Biochem. 76, 652-659 (2012)


8) Nakase M, Tani M, and Takegawa K:

Overexpression of the budding yeast IPT1 gene produces mannosyldiinositol phosphorylceramide in the fission yeast cells and inhibits cell growth.

Microbiology 158, 1219-1228 (2012)


9) Matsuzawa T, Ohashi T, Nakase M, Yoritsune K, and Takegawa K:

Galactose-specific recognition system in the fission yeast Schizosaccharomyces pombe.

Trends Glycosci. Glycotechnol. 135, 24-42 (2012) Review Article


10) Matsuzawa T, Hara F, Tohda H, Uemura H, and Takegawa K:

Promotion of glycerol utilization using ethanol and 1-propanol in Schizosaccharomyces pombe.

Appl. Microbiol. Biotechnol. 95, 441-449 (2012)


11) Klionsky DJ, et al.:

Guidelines for the use and interpretation of assays for monitoring autophagy.

Autophagy 8, 445-544 (2012) Review Article


12) Mori Y, Takegawa K, and Kimura Y:

Function analysis of conserved amino acid residues in a Mn2+-dependent protein phosphatase, Pph3, from Myxococcus xanthus.

J. Biochem. 152, 269-274 (2012)


13) Mori Y, Meda M, Takegawa K, and Kimura Y:

A tyrosine phosphatase, PphA, of Myxococcus xanthus is involved in the production of exopolysaccharide.

Microbiology 158, 2546-2555 (2012)


14) Ohashi T, Fujiyama K and Takegawa K.:

Identification of novel alpha1,3-galactosyltransferase and elimination of alpha-galactose-containing glycans by disruption of multiple alpha-galactosyltransferase genes in Schizosaccharomyces pombe.

J. Biol. Chem. 287, 38866-3875 (2012)


15) 竹川 薫、松沢智彦、東田英毅:

第3章、4 オミックス情報を用いた分裂酵母の改変、「微生物を活用した新世代の有用物質生産技術」、p70-77、シーエムシー出版 (2012)



[2011]


1) Matsuzawa T, Fujita Y, Tanaka N, Tohda H, Itadani A, and Takegawa K:

New insights into galactose metabolism by Schizosaccharomyces pombe: isolation of a galactose-assimilating mutant.

J. Biosci. Bioeng. 111, 158-166 (2011)


2) Ohashi T, Nakakita S, Sumiyoshi W, Yamada N, Ikeda Y, Tanaka N, and Takegawa K:

Structural analysis of 1,3-linked galactose-containing oligosaccharides in Schizosaccharomyces pombe mutants harboring single and multiple -galactosyltransferase genes disruptions.

Glycobiology 21, 340-351 (2011)


3) Sugimoto N, Iwaki T, Chardwiriyapreecha S, Shimazu M, Sekito T, Takegawa K, and Kakinuma Y:

Atg22p, a vacuolar membrane protein involved in amino acid compartmentalization of Schizosaccharomyces pombe.

Biosci. Biotechnol. Biochem. 75, 385-387 (2011)


4) Mukaiyama H, Iwaki T, Idiris A, and Takegawa K:

Processing and maturation mechanism of carboxypeptidase Y and alkaline phosphatase in Schizosaccharomyces pombe

Appl. Microbiol. Biotechnol. 90, 203-213 (2011)


5) Hirano T, Matsuzawa T, Takegawa K, and Sato MH:

Loss-of- and gain-of-function mutations in FAB1A/B impair endomembrane homeostasis, conferring pleiotropic developmental abnormalities in Arabidopsis.

Plant Physiol. 155, 797-807 (2011)


6) Kimura Y, Mori Y, Ina Y, and Takegawa K:

Enzymatic and functional analysis of a protein phosphatase, Pph3, from Myxococcus xanthus.

J. Bacteriol. 193, 2657-2661 (2011).


7) Futagami T, Okamoto F, Hashimoto H, Fukuzawa K, Higashi K, Nazir KHMNH, Wada E, Suyama A, Takegawa K, Goto M, Nakamura K, and Furukawa K:

Enrichment and characterization of a trichloroethene-dechlorinating consortium containing multiple “Dehalococcoides” strains.

Biosci. Biotechnol. Biochem. 75, 1268-1274 (2011).


8) Hosomi A, Nakase M, and Takegawa K:

Schizosaccharomyces pombe Pep12p is required for vacuolar protein transport and vacuolar homotypic fusion.

J. Biosci. Bioeng. 112, 309-314 (2011).


9) Kimura Y, Yamashita S, Mori Y, Kitajima Y, and Takegawa K:

A Myxococcus xanthus bacterial tyrosine kinase, BtkA, is required for the formation of mature spores.

J. Bacteriol. 193, 5853-5857 (2011)


10) Futagami T, Mori K, Yamashita A, Wada S, Kajiwara Y, Takashita H, Omori T, Takegawa K, Tashiro K, Kuhara S, and Goto M:

Genome sequence of the white Koji mold, Aspergillus kawachii IFO4308 used for brewing the Japanese distilled spirit, Shochu.

Eukaryot. Cell 10, 1586-1587 (2011)


11) Matsuzawa T, Morita T, Tanaka N, Tohda H, and Takegawa K:

Identification of a galactose-specific flocculin essential for nonsexual flocculation and hyphal growth in Schizosaccharomyces pombe.

Mol. Microbiol. 82, 1531-1544 (2011)


12) 竹川 薫、佐々木真弓、東田英毅

染色体工学技術を利用した分裂酵母の有用物質生産システムの構築, 生物工学会誌 89, 521-523 (2011)


13) 竹川 薫、藤田清貴

第3編 合成 第8章 エンドAの構造から糖タンパク質合成の最適条件を探る、バイオ医薬品開発における糖鎖技術、シーエムシー出版 (2011)



[2010]


1) Idiris A, Tohda H, Kumagai H, Giga-Hama Y, and Takegawa K:

Enhanced protein secretion from the multiprotease-deletion fission yeast by modification of vacuolar protein sorting pathway.

Appl. Microbiol. Biotechnol. 85, 667-677 (2010) [PubMed]


2) Ohashi T, and Takegawa K:

Structure of the N- and O-linked oligosaccharides that show complete loss of galactose residues from gms1och1 mutant of Schizosaccharomyces pombe.

Appl. Microbiol.Biotechnol. 86, 263-272 (2010) [PubMed]


3) Hasehira K, Nakakita S, Miyanishi N, Sumiyoshi W, Hayashi S, Takegawa K, Hirabayashi J:

A comprehensive HPLC analytical system for the identification and quantification of hexoses that employs 2-aminobenzamide coupling.

J. Biochem. 147, 501-509 (2010) [PubMed]


4) Suzuki S, Matsuzawa T, Nukigi Y, Takegawa K, and Tanaka N:

Characterization of two different types of UDP-glucose/-galactose 4-epimerase involved in galactosylation in fission yeast.

Microbiology 156, 708-718 (2010) [PubMed]


5) Mukaiyama H, Tohda H, and Takegawa K:

Overexpression of protein disulfide isomerases enhances secretion of recombinant human transferrin in Schizosaccharomyces pombe.

Appl. Microbiol. Biotechnol. 86, 1135-1143 (2010) [PubMed]


6) Mukaiyama H, Nakase M, Nakamura T, Kakinuma Y, and Takegawa K:

Autophagy in the fission yeast Schizosaccharomyces pombe.

FEBS Lett. 584, 1327-1334 (2010) [PubMed] Review Article


7) Kimura Y, Kawasaki S, Yoshimoto H, and Takegawa K:

Glycine betaine biosynthesized from glycine provides an osmolyte for cell growth and spore germination during osmotic stress in Myxococcus xanthus.

J. Bacteriol. 192, 1467-1470 (2010) [PubMed]


8) Idiris A, Tohda H, Kumagai H, and Takegawa K

Engineering of protein secretion in yeast: strategies and impact on protein production.

Appl. Microbiol. Biotechnol. 86, 403-417 (2010) [PubMed] Review Article


9) Park J-H, Nishidate T, Kijima K, Ohashi T, Takegawa K, Hirata K, Nakamura Y, and Katagiri T.:

Critical roles of MUC1 glycosylation by transactivated polypeptide N-acetylgalactosaminyltransferase 6 (GALNT6) in mammary carcinogenesis.

Cancer Res. 70, 2759-2769 (2010) [PubMed]


10) Onishi M, Koga T, Hirata A, Nakamura T, Asakawa H, Shimoda C, Bahler J, Wu J-Q, Takegawa K, Tachikawa H, Pringle JR, and Fukui Y:

Role of septins in the orientation of forespore-membrane extension during sporulation in fission yeast.

Mol. Cell. Biol. 30, 2057-2074 (2010) [PubMed]


11) Nakase M, Tani M, Morita T, Kitamoto HK, Kashiwazaki J, Nakamura T, Hosomi A, Tanaka N, and Takegawa K:

Mannosylinositol phosphorylceramide is a main phospholipid component and required for proper localization of plasma membrane proteins in Schizosaccharomyces pombe.

J. Cell Sci. 123, 1578-1587 (2010) [PubMed]


12) Matsuzawa T, Ohashi T, Hosomi A, Tanaka N, Tohda H, and Takegawa K:

The gld1+ gene encoding glycerol dehydrogenase is required for glycerol metabolism in Schizosaccharomyces pombe.

Appl. Microbiol. Biotechnol. 87, 715-727 (2010)


13) Chardwiriyapreecha S, Mukaiyama H, Sekito T, Iwaki T, Takegawa K, and Kakinuma Y:

Avt5p is required for vacuolar uptake of amino acids in the fission yeast Schizosaccharomyces pombe.

FEBS Lett. 584, 2339-2345 (2010)


14) Morita T, Ito E, Kitamoto HK, Fukuoka T, Imura T, Takegawa K, and Kitamoto D:

Identification of a gene PaEMT1 for biosynthesis of mannosylerythritol lipids in the basidiomycetous yeast Pseudozyma antarctica.

Yeast 27, 905-917 (2010)


15) Sugimoto N, Iwaki T, Chardwiriyapreecha M, Shimazu S, Sekito T, Takegawa K, and Kakinuma Y:

Vba2p: A Vacuolar Membrane Protein Involving in Basic Amino Acid Transport in Schizosaccharomyces pombe.

Biosci. Biotechnol. Biochem. 74, 2166-2169 (2010)


16) Ohashi T, Nakakita S, Sumiyoshi W, and Takegawa K:

Production of heterologous glycoproteins by a glycosylation-defective alg3och1 mutant of Schizosaccharomyces pombe.

J. Biotechnol. 150, 348-356 (2010)


[2009]

1) Ikeda, Y., Ohashi, T., Tanaka, N., and Takegawa, K.: Identification and characterization of a gene required for alpha1,2-mannose extension in the O-linked glycan synthesis pathway in Schizosaccharomyces pombe. FEMS Yeast Res 9, 115-125 (2009) [PubMed]

2) Kimura, Y., Kakemizu, A., Matsubara, Y., and Takegawa, K. Enzymatic characteristics of a Ser/Thr protein kinase, SpkA, from Myxococcus xanthus. J. Biosci. Bioeng. 107, 10-15 (2009) [PubMed]

3) Ohashi, T., Ikeda, Y., Tanaka, N., Nakakita, S., Natsuka, S., Giga-Hama, Y., and Takegawa, K. The och1 mutant of Schizosaccharomyces pombe produces galactosylated core structures of N-linked oligosaccharides. Biosci. Biotechnol. Biochem. 73407-414 (2009) [PubMed]

4) Kimura, Y., Okazaki, N., and Takegawa, K. Enzymatic characteristics of two novel Myxococcus xanthus enzymes, PdeA and PdeB, displaying 3', 5'- and 2', 3'-cAMP phosphodiesterase, and phosphatase activities. FEBS Lett. 583, 443-448 (2009) [PubMed]

5) Kashiwazaki, J., Iwaki, T., Takegawa, K., Shimoda, C., and Nakamura, T. Two Schizosaccharomyces pombe Rab7 homologs, Ypt7 and Ypt71, play antagonistic roles in the regulation of vacuolar morphology. Traffic 10, 912-924 (2009) [PubMed]

6) Nakakita, S., Hasehira, K., Hosokawa, T., Tokuda, M., Izumori, K., Takegawa, K., and Hirabayashi, J. Development of a high-sensitivity chromatographic separation system for pyridylaminated aldopentoses and aldohexoses. J. Chromatog. A. 1216, 5112-5115 (2009) [PubMed]

7) Takegawa, K., Tohda, H., Sasaki, M., Idiris, A., Ohashi, T., Mukaiyama, H., Giga-Hama, Y., and Kumagai, H. Production of heterologous proteins using the fission-yeast (Schizosaccharomyces pombe) expression system. Biotechnol Appl Biochem. 53, 227-235 (2009) [PubMed] Review article

8) Mukaiyama, H., Tohda, H., Giga-Hama, Y., and Takegawa, K. Dextran sodium sulfate is effective enhancer for secretion of recombinant human transferrin in Schizosaccharomyces pombe. Appl. Microbiol. Biotechnol. 85, 155-164 (2009) [PubMed]

9) Rothlisberger, S., Jourdain, I., Johnson, C., Takegawa, K., and Hyams, J.S. The dynamin-related protein Vps1 regulates vacuole fisson, fusion and tubulation in the fission yeast, S. pombe. Fungal Genet. Biol. 46, 927-935 (2009) [PubMed]

10) Goto, M., Harada, Y., Oka, T., Matsumoto, S., Takegawa, K., and Furukawa, K. Protein O-mannosyltransferase B and C support hyphal development and differentiation in Aspergillus nidulans. Eukaryot. Cell , 8, 1465-1474 (2009) [PubMed]

11) Mukaiyama, H., Kajiwara, S., Hosomi, A., Giga-Hama, Y., Tanaka, N., Namkamura, T., and Takegawa, K. Autophagy-deficient Schizosaccharomyces pombe mutants undergo partial sporulation during nitrogen starvation. Microbiology 155, 3816-3826 (2009) [PubMed]


12) Kimura, Y., Kakemizu, A., Matsubara, Y., and Takegawa, K. Interaction between a Ser/Thr protein kinase, SpkA, and a cAMP-dependent protein linase regulatory subunit homolog, CpbB, from Myxococcus xanthus. J. Gen. Appl. Micobiol. 55, 499-502 (2009) [PubMed]


[2008]

1) Nakayama S, Kosaka T, Hirakawa H, Matsuura K, Yoshino S, Furukawa K. (2008) Metabolic engineering for solvent productivity by downregulation of the hydrogenase gene cluster hupCBA in Clostridium saccharoperbutylacetonicum strain N1-4. Appl. Microbiol. Biotechnol., 78, 483-493.


2) Futagami, T., Goto, M., and Furukawa, K. (2008) Biochemical and genetic bases of dehalorespiration. Chem. Rec., 8, 1-12.


3) Takanashi, K., Shitan, N., Sugiyama, A., Kamimoto, Y., Hamamoto, M., Iwaki, T., Takegawa, K. and Yazaki, K. (2008) Galactinol synthase genes of Coptis japonica are involved in confers berberine tolerance in yeast. Biosci. Biotechnol. Biochem. 72, 398-405.


4) Hosomi, A., Kawanishi, Y., Tanaka, N., and Takegawa, K. (2008) PXA domain-containing protein Pxa1 is required for normal vacuole function and morphology in Schizosaccharomyces pombe. Biosci. Biotechnol. Biochem. 72, 548-556.


5) Iwaki, T., Iefuji, H., Hiraga, Y., Hosomi, A., Morita, T., Giga-Hama, Y., and Takegawa, K. (2008) Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe. Microbiology 154, 830-841.


6) Li, B., Takegawa, K., Suzuki, T., Yamamoto, K., and Wang, L.-X. (2008) Synthesis and inhibitory activity of oligosaccharide thiazolines as a class of mechanism-based inhibitors for endo-beta-N-acetylglucosaminidases. Bioorg. Med. Chem. 16. 4670-4675.


7) Chardwiriyapreecha, S., Shimazu, M., Morita, T., Sekito, T., Akiyama, K., Takegawa, K., and Kakinuma, Y. (2008) Identification of the fnx1+ and fnx2+ genes for vacuolar amino acid transporters in Schizosaccharomyces pombe. FEBS Lett. 582, 2225-2230.


[2007]

1) Goto, M. (2007) Protein O-glycosylation in fungi; diverse structures and multiple functions. Biosci. Biotechnol. Biochem., 71, 1415-1427. Review article


2) Nakayama, S., Irie, R., Kosaka, T., Matsuura, K., Yoshino, S., and Furukawa, K. (2007) New host-vector system in solvent-producing Clostridium saccharoperbutylacetonicum strain N1-4. J. Gen. Appl. Microbiol., 53, 53-56.


3) Kosaka, T., Nakayama, S., Nakaya, K., Yoshino, S., and Furukawa, K. (2007) Characterization of the sol operon in butanol-hyperproducing Clostridium saccharoperbutylacetonicum strain N1-4 and its degeneration mechanism. Biosci. Biotechnol. Biochem. 71, 58-68.


4) Yamamoto, K., Ishimaru, I., Fujii, Y., Kuriyama, S., Masaki, T., Takegawa, K., and Tanaka, N. (2007) Method for measuring the three dimensional distribution of a fluorescent dye in a cell membrane. Appl. Phys. Lett. 90, 021110.


5) Giga-Hama, Y., Tohda, H., Takegawa, K., and Kumagai, H. (2007) Minimum genome factory using fission yeast Schizosaccharomyces pombe, improvement of host genome for heterologous protein production. Biotechnol. Appl. Biochem. 46, 147-155. Review article


6) Iwaki, T., Morita, T., Tanaka, N., Giga-Hama, Y., and Takegawa, K. (2007) Loss of a GPI-anchored membrane protein Aah3p causes a defect of vacuolar protein sorting in Schizosaccharomyces pombe. Biosci. Biotechnol. Biochem. 71, 623-626.


7) Yasokawa, T., Ishizaki, T., K. Ishizaki, K. Gesho, Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N. (2007) Technique for measuring the rotational velocity of a single cell. Appl. Phys. Lett. 90, 051103.


8) Miyatake, M., Kuno, T., Kita, A., Katsura, K., Takegawa, K., Nabata, T., and Sugiura, R. (2007) Valpronic acid affects trafficking and cell wall integrity in fission yeast. Genetics 175, 1695-1705.


9) Iwaki, T., Onishi, M., Ikeuchi, M., Kita, A., Sugiura, R., Giga-Hama, Y., Fukui, Y., and Takegawa, K. (2007) Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe. Microbiology 153, 2753-2764.


10) Yasokawa, T., Ishimaru, I., Kondo, M., Kuriyama, S., Masaki, T., Takegawa, K., and Tanaka, N. (2007) A method for measuring the three-dimensional refractive-index distribution of single cells using proximal two-beam optical tweezers and a phase-shifting Mach-Zehnder interferometer. Opt. Rev. 14, 161-164.


11) Fujita, K., Sato, R., Toma, K., Yamamoto, K., and Takegawa, K. (2007) Identification of the catalytic acid-base residue of Arthrobacter endo-beta-N-acetylglucosaminidase by chemical rescue of inactive mutant. J. Biochem. 142, 301-306.


12) Ma, Y., Sugiura, R., Saito, M., Koike, A., Sio, S.-O., Fujita, Y., Takegawa, K. and Kuno, T. (2007) New amino acid-auxotrophic markers for targeted gene integration and disruption in fission yeast Curr.Genet. 52, 97-105.


13) Yasokawa, T., Ishimaru, I., Kuriyama, S., Masaki, T., Takegawa, K., and Tanaka, N. (2007) A double filtering method for measuring the translational velocity of fluorescently stained cells. Appl. Phys. Lett. 91, 131116.


14) Onishi, M., Iida, M., Koga, T., Yamada, S., Hirata, A., Iwaki, T., Takegawa, K., Fukui, Y., and Tachikawa, H. (2007) Fission yeast Sst4p, a conserved Vps27/Hrs homolog, functions downstream of PtdIns 3-kinase Pik3p to mediate proper spore formation. Eukaryot. Cell 6, 2343-2353.


15) 二神泰基,後藤正利,古川謙介. 脱ハロゲン呼吸細菌の還元的デハロゲナーゼ. J. Environ. Biotechnol., 7(2), 107-110 (2007)


16) 後藤正利. 糸状菌におけるO-グリコシレーションの役割.バイオサイエンスとインダストリー, 65(5), 30-31 (2007)


17) 後藤正利,二神泰基,藤原秀彦,古川謙介.難分解性化学物質の微生物による分解・無毒化技術有機物の分解・無害化技術 最前線第2章第4節, pp.291-304,情報機 (2007


18) 二神泰基,古川謙介.微生物による環境汚染有機塩素化合物の分解.排水・汚水処理技術集成 第2章第7節, pp. 130-138, NTS出版 (2007


19) 浜 裕子、竹川 薫.4章 分裂酵母のミニマムゲノムファクトリー、微生物機能を活用した革新的生産技術の最前線、p49-61, シーエムシー出版 (2007)


20) 竹川 薫 第III章 3.10 液胞、酵母のすべて、p78-83, シュプリンガージャパン (2007)


21) 竹川 薫 第3章 微生物遺伝学と遺伝子工学、遺伝子から見た応用微生物学 p30-50, 朝倉書店(2007


[2006]

1) Futagami, T., Yamaguchi, T., Nakayama, S., Goto, M., and Furukawa, K. (2006) Effects of chloromethanes on growth of and deletion of the pce gene cluster in dehalorespiring Desulfitobacterium hafniense strain Y51. Appl. Environ. Microbiol. 72, 5998-6003.


2) Fujihara, H., Yoshida, H., Matsunaga, T., Goto, M., and Furukawa, K. (2006) Cross-regulation of biphenyl- and salicylate-catabolic genes by two regulatory systems in Pseudomonas pseudoalcaligenes KF707. J. Bacteriol. 188, 4690-4697.


3) Suenaga, H., Goto, M., and Furukawa, K. (2006) Active-site engineering of biphenyl dioxygenase: effect of substituted amino acids on substrate specificity and regiospecificity. Appl. Microbiol. Biotechnol., 71, 168-176.


4) Futagami, T., Tsuboi, Y., Suyama, A., Goto, M., and Furukawa, K. (2006) Emergence of two types of nondechlorinating variants in the tetrachloroethene-halorespiring Desulfitobacterium sp. strain Y51. Appl. Microbiol. Biotechnol., 70, 720-728.


5) Suenaga,H., Sato, M., Goto, M., Takeshita, M., and Furukawa, K. (2006) Steady-state kinetic characterization of evolved biphenyl dioxygenase, which acquired novel degradation ability for benzene and toluene. Biosci. Biotechnol. Biochem., 70, 1021-1025.


6) Fujita, Y., Ukena, E., Iefuji, H., Giga-Hama, Y., and Takegawa, K.(2006) Homocysteine accumulation causes a defect in purine biosynthesis: further characterization of Schizosaccharomyces pombe methionine auxotrophs. Microbiology 152, 397-404.


7) Haneda, K., Takeuchi, M., Tagashira, M., Inazu, T., Toma, K., Isogai, Y., Hori, M., Kobayashi, K., Takeuchi, M., Takegawa, K., and Yamamoto, K. (2006) Chemo-enzymatic synthesis of eel calcitonin glycosylated at two sites with the same and different carbohydrate structures. Carbohydr. Res. 341, 181-190.


8) He, Y., Sugiura, R., Ma, Y., Kita, A., Deng, L., Takegawa, K., Matsuoka, K., Shuntoh, H., and Kuno, T. (2006) Genetic and functional interaction between Ryh1 and Ypt3: two Rab GTPases that function in S. pombe secretory pathway. Genes Cells 11, 207-221.


9) Hirashima, K., Iwaki, T., Takegawa, K., Giga-Hama, Y., and Tohda, H. (2006) A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast. Nucleic Acids Res. 34, e11.


10) Morita, T., Tanaka, N., Hosomi, A., Giga-Hama, Y., and Takegawa, K. (2006) An alpha-amylase homologue, aah3+, encodes a GPI-anchored membrane protein required for cell wall integrity in Schizosaccharomyces pombe. Biosci. Biotechnol. Biochem. 70, 1454-1463.


11) Iwaki, T., Hosomi, A., Tokudomi, S., Kusunoki, Y., Fujita, Y., Giga-Hama, Y., Tanaka, N., and Takegawa, K. (2006) Vacuolar protein sorting receptor in Schizosaccharomyces pombe. Microbiology 152, 1523-1532.


12) Fujita, Y., Tohda, H., Giga-Hama, Y., and Takegawa, K. (2006) Heat shock-inducible expression vectors for use in Schizosaccharomyces pombe.FEMS Yeast Res. 6, 883-887.


13) Ishizaki, K., Ishimaru, I., Yoshida, M., Inoue, Y., Yasokawa, T., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N. (2006) Translational velocity measurement for single floating cell based on optical Fourier transform theory. Appl. Phys. Lett. 88, 101114.


14) Kobayashi, H., Ishimaru, I., Hyodo, R., Yasokawa, T., Ishizaki, T., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N. (2006) A pricise method for rotating single cells. Appl. Phys. Lett. 88, 131103.


15) Mai, H.T., Nomura, M., Takegawa, K., Asamizu E., Sato S., Kato T., Tabata S., and Tajima, S. (2006) Identification of a Sed5-like SNARE gene LjSYP32-1 that contributes to nodule tissue formation of Lotus japonicus. Plant Cell Physiol. 47, 829-838.


16) Iwaki, T., Giga-Hama, Y., and Takegawa, K. (2006) A survey of all 11 ABC transporters in fission yeast: two novel ABC transporters are required for red pigment accumulation in a Schizosaccharomyces pombe adenine biosynthetic mutant. Microbiology 152, 2309-2321.


17) Inoue, Y., Ishimaru, I., Yasokawa, T., Ishizaki, K., Yoshida, M., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N. (2006) Variable phase-contrast fluorescence spectrometry for fluorescently stained cells. Appl. Phys. Lett. 89, 121103.


18) Yasokawa, T., Ishimaru, I., Nakagawa, T., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N. (2006) Attitudinal manipulation of an optically trapped bacillary probe by controlling the distance between focal points for local dosing in cells. Appl. Phys. Lett. 89, 131107.


19) Yoshida, M., Ishimaru, I., Ishizaki, K., Yasokawa, T., Kuriyama, S., Masaki, T., Takegawa, K., and Tanaka, N. (2006) Displacement measurement of the depth migration of transparent cells. Appl. Phys. Lett. 89, 241102.


20) Kobayashi, H., Ishimaru, I., Yasokawa, T., Ishizaki, K., Kuriyama, S., Masaki, T., Nakai, S., Takegawa, K., and Tanaka, N. (2006) Three dimentional phase-contrast Imaging for single floating cells. Appl. Phys. Lett. 89, 241117.


21) Takegawa, K. (2006) Cloning of Endoglycosidases, Endoglycosidases - Biochemistry, Biotechnology, Application. Endo, M., Yamamoto, K., Takagaki, K., and Hase, S. eds. p44-51, Springer


22) Takegawa, K. (2006) Endo-b-N-acetylglucosaminidase, Endoglycosidases - Biochemistry, Biotechnology, Application. Endo, M., Yamamoto, K., Takagaki, K., and Hase, S. eds. p69-74, Springer


23) Takegawa, K. (2006) 3.1.3 Enzymatic Synthesis of Neoglycoconjugates Using Oligosaccharide-transfer Activity of Endo-A, Endoglycosidases - Biochemistry, Biotechnology, Application. Endo, M., Yamamoto, K., Takagaki, K., and Hase, S. eds. p149-154, Springer


24) Takegawa, K. (2006) 3.2.1 Remodeling of Sugar Chains by Endo-A, Endoglycosidases - Biochemistry, Biotechnology, Application. Endo, M., Yamamoto, K., Takagaki, K., and Hase, S. eds. p154-159, Springer


25)竹川 薫 分裂酵母のゲノム情報を活用した異種タンパク質生産、化学と生物、44, 241-246 (2006)



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