Publications

2024

Akira Tsukamoto, Lee Jae Man, Kosuke Oyama, Akitsu Masuda, Hiroaki Mon, Tadashi Ueda, Takahiro Kusakabe
Effective expression and characterization of the receptor binding domains in SARS-CoV-2 Spike proteins from original strain and variants of concern using Bombyx mori nucleopolyhedrovirus in silkworm
Protein Expression and Purification, 2024, 218:106450.

2023

Kohei Kakino, Hiroaki Mon, Takeru Ebihara, Masato Hino, Akitsu Masuda, Jae Man Lee, Takahiro Kusakabe
Comprehensive Transcriptome Analysis in the Testis of the Silkworm, Bombyx mori
Insects, 2023, 14:8:684.

Takeyuki Goto, Tomoki Sasaki, Yong Chong, Masahiro Taniguchi, Jae Man Lee, Akitsu Masuda, Takeru Ebihara, Kenichiro Shiraishi, Naoki Tani, Akiko Yonekawa, Kei Gondo, Hiroyuki Kuwano, Nobuyuki Shimono, Hideyuki Ikematsu, Koichi Akashi, Takahiro Kusakabe
SARS-CoV-2 strain-specific anti-Spike IgG ELISA utilizing spike protein produced by silkworms
Human Antibodies, 2023, 31:3:27-33.

Akihiro Morio, Jae Man Lee, Tsuguru Fujii, Hiroaki Mon, Akitsu Masuda, Kohei Kakino, Jian Xu, Yutaka Banno, Takahiro Kusakabe
The biological role of core 1β1-3galactosyltransferase (T-synthase) in mucin-type O-glycosylation in Silkworm, Bombyx mori
Insect Biochemistry and Molecular Biology, 2023, 156:103936.

Keisuke Tanaka, Kosuke Minamihata, Rie Wakabayashi, Jae Man Lee, Takeshi Miyata, Takahiro Kusakabe, Noriho Kamiya, Masahiro Goto
Transdermal Transmission Blocking Vaccine for Malaria using a Solid-in-Oil Dispersion
Journal of Pharmaceutical Sciences, 2023, 112:2:411-415.

Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Shintaro Sato, Atsushi Masuda, Masahiro Taniguchi, Ryosuke Fujita, Hiroshi Ushijima, Keisuke Morimoto, Takeru Ebihara, Masato Hino, Kohei Kakino, Hiroaki Mon, Takahiro Kusakabe
High yield production of norovirus GII.4 virus-like particles using silkworm pupae and evaluation of their protective immunogenicity
Vaccine, 2023, 41:3:766-777.

Yuri Kato, Kazuhiro Nishiyama, Jae Man Lee, Yuko Ibuki, Yumiko Imai, Takamasa Noda, Noriho Kamiya, Takahiro Kusakabe, Yasunari Kanda, Motohiro Nishida
TRPC3-Nox2 Protein Complex Formation Increases the Risk of SARS-CoV-2 Spike Protein-Induced Cardiomyocyte Dysfunction through ACE2 Upregulation
International Journal of Molecular Sciences, 2023, 24:1:102.

2022

Takeshi Miyata, Kosuke Minamihata, Koichi Kurihara, Yui Kamizuru, Mari Gotanda, Momoka Obayashi, Taiki Kitagawa, Keita Sato, Momoko Kimura, Kosuke Oyama, Yuta Ikeda, Yukihiro Tamaki, Jae Man Lee, Kozue Sakao, Daisuke Hamanaka, Takahiro Kusakabe, Mayumi Tachibana, Hisham R Ibrahim
Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25, by silkworm and its biochemical analysis
Protein Expression and Purification, 2022, 195-196:106096.

Masato Hino, Tsuneyuki Tatsuke, Akihiro Morio, Hiroaki Mon, Jae Man Lee, Akitsu Masuda, Kohei Kakino, Yoshino Tonooka, Takahiro Kusakabe
Characterization of a Novel Heterochromatin Protein 1 Homolog “HP1c” in the Silkworm, Bombyx mori
Insects, 2022, 13:7:631.

Miyu Tanaka, Tsuguru Fujii, Hiroaki Mon, Jae Man Lee, Kohei Kakino, Hisayoshi Fukumori, Takeru Ebihara, Takumi Nagasato, Masato Hino, Yoshino Tonooka, Takato Moriyama, Ryosuke Fujita, Yutaka Banno, Takahiro Kusakabe
Silkworm FoxL21 plays important roles as a regulator of ovarian development in both oogenesis and ovariole development
Insect biochemistry and molecular biology, 2022, 143:103737.

Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Hiroaki Mon, Keita Sato, Kosuke Oyama, Yasuteru Sakurai, Jiro Yasuda, Daisuke Takahashi, Tadashi Ueda, Yuri Kato, Motohiro Nishida, Noriko Karasaki, Kohei Kakino, Takeru Ebihara, Takumi Nagasato, Masato Hino, Ayaka Nakashima, Kengo Suzuki, Yoshino Tonooka, Miyu Tanaka, Takato Moriyama, Hirokazu Nakatake, Ryosuke Fujita, Takahiro Kusakabe
Optimization of SARS-CoV-2 Spike Protein Expression in the Silkworm and Induction of Efficient Protective Immunity by Inoculation With Alum Adjuvants
Frontiers in Immunology, 2022, 12:803647.

    2021

    Hirofumi Ohga, Kosuke Ito, Kohei Kakino, Hiroaki Mon, Takahiro Kusakabe, Jae Man Lee, Michiya Matsuyama
    Leptin Is an Important Endocrine Player That Directly Activates Gonadotropic Cells in Teleost Fish, Chub Mackerel
    CELLS, 2021, 10:12:3505.

    Tsuguru Fujii, Kohei Kakino, Hisayoshi Fukumori, Masato Hino, JaeMan Lee, Takahiro Kusakabe, Yutaka Banno
    Non-molting dwarf (nm-d) as a mutant of Bombyx mori with a defect in purine synthesis
    Insect Biochemistry and Molecular Biology, 2021, 138:103636.

    Takeru Ebihara, Akitsu Masuda, Daisuke Takahashi, Masato Hino, Hiroaki Mon, Kohei Kakino, Tsuguru Fujii, Ryosuke Fujita, Tadashi Ueda, Jae Man Lee, Takahiro Kusakabe
    Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System
    Molecular Biotechnology, 2021, 63:12:1223-1234.

    Akitsu Masuda, Jae Man Lee, Takeshi Miyata, Takeru Ebihara, Kohei Kakino, Masato Hino, Ryosuke Fujita, Hiroaki Mon, Takahiro Kusakabe
    Stable trimer formation of spike protein from porcine epidemic diarrhea virus improves the efficiency of secretory production in silkworms and induces neutralizing antibodies in mice
    Veterinary Research, 2021, 52:1:102-102.

    Takeru Ebihara, Jian Xu, Yoshino Tonooka, Takumi Nagasato, Kohei Kakino, Akitsu Masuda, Kosuke Minamihata, Noriho Kamiya, Hirokazu Nakatake, Yuuka Chieda, Hiroaki Mon, Tsuguru Fujii, Takahiro Kusakabe, Jae Man Lee
    Active Human and Murine Tumor Necrosis Factor α Cytokines Produced from Silkworm Baculovirus Expression System
    Insects, 2021, 12:6:517.

    Ryo Nagai, Takeru Ebihara, Kohei Kakino, Akitsu Masuda, Jian Xu, Kosuke Minamihata, Noriho Kamiya, Tatphon Kongkrongtong, Masahiro Kawahara, Hiroaki Mon, Tsuguru Fujii, Takahiro Kusakabe, Jae Man Lee
    Production of an active Mus musculus IL-3 using updated silkworm-based baculovirus expression vector system
    Journal of Asia-Pacific Entomology, 2021, 24:2:544-549.

      2020

      Masato Hino, Noriko Karasaki, Tsuneyuki Tatsuke, Daisuke Morokuma, Akitsu Masuda, Hiroaki Mon, Jae Man Lee, Takahiro Kusakabe
      Mild inactivation and inhibition of phenoloxidase in silkworm serum for xeno-free culture of insect cells
      Journal of Insect Biotechnology and Sericology, 2020, 89:9-16.

      Tsuguru Fujii, Kohei Kakino, Miyu Tanaka, Jae Man Lee, Takahiro Kusakabe, Yutaka Banno
      A defect in purine nucleotide metabolism in the silkworm, Bombyx mori, causes a translucent larval integument and male infertility
      Insect Biochemistry and Molecular Biology, 2020, 126:103458.

      Ryosuke Fujita, Masato Hino, Takeru Ebihara, Takumi Nagasato, AkitsuMasuda, Jae Man Lee, Tsuguru Fujii, Hiroaki Mon, Kohei Kakino, Ryo Nagai, Miyu Tanaka, Yoshino Tonooka, Takato Moriyama, Takahiro Kusakabe
      Efficient production of recombinant SARS-CoV-2 spike protein using the baculovirus-silkworm system
      Biochem Biophys Res Commun, 2020, 529:257-262.

      Kohei Kakino, Akitsu Masuda, Masato Hino, Takeru Ebihara, Jian Xu, Hiroaki Mon, Ryosuke Fujita, Tsuguru Fujii, Takahiro Kusakabe, Jae Man Lee
      Efficient production of recombinant T7 endonuclease I using silkworm-baculovirus expression vector system
      Journal of Asia-Pacific Entomology, 2020, 23:694-700.

      Masahiko Kobayashi, Jian Xu, Kohei Kakino, Akitsu Masuda, Masato Hino, Naoki Fujimoto, Kosuke Minamihata, Noriho Kamiya, Hiroaki Mon, Hiroshi Iida, Masateru Takahashi, Takahiro Kusakabe, Jae Man Lee
      Optimal silkworm larva host for high-level production of Mus musculus IL-4 using a baculovirus expression vector system
      Journal of Asia-Pacific Entomology, 2020, 23:1:268-273.