海洋資源化学研究室

論文発表

原著論文 (総説はこちら）

2023年

  • Characterization of thraustochytrid-specific sterol O-acyltransferase: modification of DGAT2-like enzyme to increase the sterol production in Aurantiochytrium limacinum mh0186.
    Ishibashi Y, Sadamitsu S, Fukahori Y, Yamamoto Y, Tanogashira R, Watanabe T, Hayashi M, Ito M, Okino N
    Appl Environmen Microbiol, 89(11), e0100123. https://doi.org/10.1128/aem.01001-23
  • Lysoglycosphingolipids have the ability to induce cell death through direct PI3K inhibition.
    Watanabe, R., Tsuji, D., Tanaka, H., Uno, M. S., Ohnishi, Y., Kitaguchi, S., Matsugu, T., Nakae, R., Teramoto, H., Yamamoto, K., Shinohara, Y., Hirokawa, T., Okino, N., Ito, M., Itoh, K.
    Journal of Neurochemistry, 167, 753–765. https://doi.org/10.1111/jnc.16012.
  • Simultaneous structural replacement of the sphingoid long‐chain base and sterol in budding yeast
    Kono Y, Ishibashi Y, Fukuda S, Higuchi T, Tani M
    FEBS J, 290(23), 5605–5627.
  • Impaired biosynthesis of ergosterol confers resistance to complex sphingolipid biosynthesis inhibitor aureobasidin A in a PDR16-dependent manner.
    Fukuda S, Kono Y, Ishibashi Y, Tabuchi M, Tani M
    Scientific reports, 13(1), 11179.
  • Assays and Utilization of Enzymes Involved in Glycolipid Metabolism in Bacteria and Fungi
    Ito M, Ishibashi Y, Watanabe T, Iwaki J, Kurita T, Okino N
    Methods Mol Biol, 2613, 229–256.

2022年

  • Loss of tolerance to multiple environmental stresses due to limitation of structural diversity of complex sphingolipids
    Koga A, Takayama C, Ishibashi Y,Kono Y, Matsuzaki M, Tani M.
    Mol Biol Cell(2022) mbcE22040117. doi: 10.1091/mbc.E22-04-0117
  • Proteomic and lipidomic analyses of lipid droplets in Aurantiochytrium limacinum ATCC MYA-1381
    Yoneda K, Ishibashi Y, Yoshida M, Watanabe M. Ito M, Iwane S.
    Algal Research (2022) https://doi.org/10.1016/j.algal.2022.102844
  • Significance of mitochondrial fatty acid β-oxidation for the survivability of Aurantiochytrium limacinum ATCC MYA-1381 during sugar starvation
    Nyunoya H, Ishibashi Y, Ito M, Okino N.
    Biosci Biotechnol Biochem (2022) doi: 10.1093/bbb/zbac141.

2021年

  • PUFA synthase-independent DHA synthesis pathway in Parietichytrium sp. and its modification to produce EPA and n-3DPA
    Ishibashi Y, Goda H, Hamaguch R, Sakaguchi K, Sekiguchi T, Ishiwata Y, Okita Y, Mochinaga S, Ikeuchi S, Mizobuchi T, Takao Y, Mori K, Tashiro K, Okino N, Honda D, Hayashi Ito M.
    Commun. Biol. 4, 1378 (2021). https://doi.org/10.1038/s42003-021-02857-w
  • Oxygen plasma modulates glucosinolate levels without affecting lipid contents and composition in Brassica napus seeds
    Maruyama-Nakashita A, Ishibashi Y, Yamamoto K, Liu Z, Morikawa-Ichinose T, Kim SJ, Hayashi N
    Biosci. Biotechnol. Biochem.(2021) zbab157, https://doi.org/10.1093/bbb/zbab157
  • Crosstalk between protein kinase A and the HOG pathway under impaired biosynthesis of complex sphingolipids in budding yeast
    Urita A, Ishibashi Y, Kawaguchi R, Yanase Y, and Tani M
    FEBS J (2021). DOI: 10.1111/febs.16188, 2021.09.
  • C4-monomethylsterol β-glucoside and its synthase in Aurantiochytrium limacinum mh0186
    Endo I, Watanabe T, Miyamoto T, Monjyusho-Goda H, Ohara J, Hayashi M, Hama Y, Ishibashi Y, Okino N, Ito M
    Glycobiology, cwab070, https://doi.org/10.1093/glycob/cwab070 (2021)
  • Lack of ∆5 Desaturase Activity Impairs EPA and DHA Synthesis in Fish Cells from Red Sea Bream and Japanese Flounder
    Nyunoya H, Noda I, Kawamoto Y, Hayashi Y, Ishibashi Y, Ito M, Okino N
    Mar Biotechnol (2021). https://doi.org/10.1007/s10126-021-10040-9

2020年

  • The sirtuin inhibitor cambinol reduces intracellular glucosylceramide with ceramide accumulation by inhibiting glucosylceramide synthase
    Ishibashi Y, Ito M, Hirabayashi Y
    Biosci. Biotechnol. Biochem. https://doi.org/10.1080/09168451.2020.1794785 (2020)
  • Two bacterial glycosphingolipid synthases responsible for the synthesis of glucuronosylceramide and α-galactosylceramide
    Okino N, Li M, Qu Q, Nakagawa T, Hayashi Y, Matsumoto M, Ishibashi Y, Ito M
    J. Biol. Chem. jbc.RA120.013796. doi:10.1074/jbc.RA120.013796 (2020)
  • Determination of globotriaosylceramide analogs in the organs of a mouse model of Fabry disease
    Ishii S, Taguchi A, Okino N, Ito M, Maruyama H
    J. Biol. Chem in press doi:10.1074/jbc.RA120.012665 (2020)

2019年

  • A thraustochytrid-specific lipase/phospholipase with unique positional specificity contributes to microbial competition and fatty acid acquisition from the environment
    Ishibashi Y, Aoki K, Okino N, Hayashi M, Ito M
    Scientific Reports volume 9, Article number: 16357 (2019)
  • Expression, purification, and characterization of highly active endo-α-N-acetylgalactosaminidases expressed by silkworm-baculovirus expression system.
    Masuda, A., Kinoshita, Y., Hino, M., Morokuma, D., Goda, H. M., Okino, N., Ito, M., Mon, H., Fujita, R., Kusakabe, T., and Lee, J. M.
    Journal of Asia-Pacific Entomology 22, 404-408 (2019)
  • The glycerol-3-phosphate acyltransferase PLAT2 functions in the generation of DHA-rich glycerolipids in Aurantiochytrium limacinum F26-b.
    Nutahara E, Abe E, Uno S, Ishibashi Y, Watanabe T, Hayashi M, Okino N, Ito M
    PLoS One. 2019 Jan 30;14(1):e0211164.

2018年

  • Lactosylceramide synthases encoded by B4galt5 and 6 genes are pivotal for neuronal generation and myelin formation in mice.
    Yoshihara T, Satake H, Nishie T, Okino N, Hatta T, Otani H, Naruse C, Suzuki H, Sugihara K, Kamimura E, Tokuda N, Furukawa K, Fururkawa K, Ito M, Asano M
    PLoS Genet. 16;14(8):e1007545. (2018)
  • Regulation of Glucosylceramide Synthesis by Golgi-localized Phosphoinositide
    Ishibashi Y, Ito M, Hirabayashi Y
    Biochem. Biophys. Res. Commun. 499:1011-1018. (2018)
  • Draft Genome Sequence of Sphingobacterium sp. Strain HMA12, Which Encodes Endo-β-N-Acetylglucosaminidases and Can Specifically Hydrolyze Fucose-Containing Oligosaccharides
    Huang Y, Higuchi Y, Mori K, Yamashita R, Okino N, Tashiro K, Takegawa K
    Genome Announc. 2018 Feb 22;6(8). pii: e01525-17. doi: 10.1128/genomeA.01525-17.
  • Visualization of endoplasmic reticulum and mitochondria in Aurantiochytrium limacinum by the expression of EGFP with cell organelle-specific targeting/retaining signals.
    Okino N, Wakisaka H, Ishibashi Y, Ito M
    Marine Biotechnology 20(2), 182-192(2018)

2017年

  • Regulation of triacylglycerol accumulation and lipid droplet morphology by the novel thraustochytrid-specific lipid droplet protein 1 (TLDP1) in Aurantiochytrium limacinum F26-b.
    Watanabe T, Sakiyama1 R, Iimi Y, Sekine S, Abe E, Nomura K, Nomura K, Ishibashi Y, Okino N, Hayashi M, Ito M
    J. Lipid Res. 58:2334-2347(2017)
  • Enhancing the sialylation of recombinant EPO produced in CHO cells via the inhibition of glycosphingolipid biosynthesis.
    Kwak CY, Park SY, Lee CG, Okino N, Ito M, Kim JH
    Sci. Rep. 7, 13059 (2017)

2016年

  • Molecular mechanism for sphingosine-induced Pseudomonas ceramidase expression through the transcriptional regulator SphR.
    Okino N, Ito M
    Sci. Rep. 6, 38797(2016)
  • A label-free impedance-based whole cell assay revealed a new G protein-coupled receptor ligand for mouse microglial cell migration.
    Fukano Y, Okino N, Furuya S, Ito M
    Biochem. Biophys. Res. Commun. 478:624-630.(2016)
  • Cell-free methods to produce structurally intact mammalian membrane proteins.
    Shinoda T, Shinya N, Ito K, Ishizuka-Katsura Y, Ohsawa N, Terada T, Hirata K, Kawano Y, Yamamoto M, Tomita T, Ishibashi Y, Hirabayashi Y, Kimura-Someya T, Shirouzu M, Yokoyama S
    Sci. Rep. Article number: 30442 (2016)

2015年

  • Ergosteryl-β-glucosidase (Egh1) involved in sterylglucoside catabolism and vacuole formation in Saccharomyces cerevisiae.
    Watanabe T, Tani M, Ishibashi Y, Endo I, Okino N, Ito M
    Glycobiology 25(10): 1079-1089 (2015)
  • AMP-activated Protein Kinase Suppresses Biosynthesis of Glucosylceramide by Reducing Intracellular Sugar Nucleotides.
    Ishibashi Y, Hirabayashi Y
    J .Biol .Chem. 290(29): 18245-18260 (2015)
  • Sterylglucoside catabolism in Cryptococcus neoformans with endoglycocoramidase-related protein2(EGCrP2), the first steryl-β-glucosidase identifed in fungi.
    Watanabe T, Ito T, Goda HM, Ishibashi Y, Miyamoto T, Ikeda K, Taguchi R, Okino N, Ito M
    J. Biol. Chem. 290(2): 1005-1019 (2015)
  • Fluorimetric assays for N-acetylgalactosamine-6-sulfatase and arylsulfatase B based on the natural substrates for confirmation of mucopolysaccharidoses types IVA and VI.
    Kumar AB, Spacil Z, Ghomashchi F, Masi S, Sumida T, Ito M, Turecek F, Scott CR, Gelb MH
    Clin. Chim. Acta. 451(Pt B):125-128 (2015)
  • Tandem Mass Spectrometry Has a Larger Analytical Range than Fluorescence Assays of Lysosomal Enzymes: Application to Newborn Screening and Diagnosis of Mucopolysaccharidoses Types II, IVA, and VI.
    Kumar AB, Masi S, Ghomashchi F, Chennamaneni NK, Ito M, Scott CR, Turecek F, Gelb MH, Spacil Z
    Clin. Chem. 61(11):1363-1371 (2015)

2014年

  • Pathological roles of the VEGF/SphK pathway in Niemann-Pick type C neurons.
    Lee H, Lee JK, Park MH, Hong YR, Marti HH, Kim H, Okada Y, Otsu M, Seo EJ, Park JH, Bae JH, Okino N, He X, Schuchman EH, Bae JS, Jin HK
    Nat. Commun. 5:5514(2014)
  • A novel ether-linked phytol-containing digalactosylglycerolipid in the marine green alga, Ulva pertusa.
    Ishibashi Y, Nagamatsu Y, Miyamoto T, Matsunaga N, Okino N, Yamaguchi K, Ito M
    Biochem. Biophys. Res. Commun. 452:873-880. (2014)
  • Novel Lysophospholipid Acyltransferase PLAT1 of Aurantiochytrium limacinum F26-b Responsible for Generation of Palmitate-Docosahexaenoate-Phosphatidylcholine and Phosphatidylethanolamine.
    Abe E, Ikeda K, Nutahara E, Hayashi M, Yamashita A, Taguchi R, Doi K, Honda D, Okino N, Ito M
    PLoS ONE 9(8): e102377 (2014)
  • Identification of perfluorooctane sulfonate binding protein in the plasma of tiger pufferfish Takifugu rubripes.
    Honda M, Muta A, Akasaka T, Inoue Y, Shimasaki Y, Kannan K, Okino N, Oshima Y.
    Ecotoxicol. Environ. Saf. 104: 409-413. (2014)
  • Pseudomonas-derived ceramidase induces production of inflammatory mediators from human keratinocytes via sphingosine-1-phosphate.
    Oizumi A, Nakayama H, Okino N, Iwahara C, Kina K, Matsumoto R, Ogawa H, Takamori K, Ito M, Suga Y, Iwabuchi K.
    PLoS One. 9(2): e89402 (2014)
  • Inhibition of GM3 Synthase Attenuates Neuropathology of Niemann-Pick Disease Type C by Affecting Sphingolipid Metabolism.
    Lee H, Lee JK, Bae YC, Yang SH, Okino N, Schuchman EH, Yamashita T, Bae JS, Jin HK.
    Mol. Cells 37(2): 161-171. (2014)
  • Elevated water temperature reduces the acute toxicity of the widely used herbicide diuron to a green alga, Pseudokirchneriella subcapitata.
    Tasmin R, Shimasaki Y, Tsuyama M, Qiu X, Khalil F, Okino N, Yamada N, Fukuda S, Kang IJ, Oshima Y.
    Environ. Sci. Pollut. Res. Int. 21(2): 1064-1070 (2014)

2013年

  • Two fatty acid elongases possessing C18-D6/C18-D9/C20-D5 or C16-D9 elongase activity in Thraustochytrium sp. ATCC26185.
    Ohara J, Sakaguchi K, Okita Y, Okino N, Ito M.
    Marine Biotechnology 15(4): 476-486 (2013)
  • Total cellular glycomics allows characterizing cells and streamlining the discovery process for cellular biomarkers.
    Fujitani N, Furukawa J, Araki K, Fujioka T, Takegawa Y, Piao J, Nishioka T, Tamura T, Nikaido T, Ito M, Nakamura Y, Shinohara Y.
    Proc. Natl. Acad. Sci. USA 110 (6): 2105–2110 (2013)
  • Vibrios adhere to epithelial cells in the intestinal tract of red sea bream, Pagrus major, utilizing GM4 as an attachment site.
    Chisada SI, Shimizu K, Kamada H, Matsunaga N, Okino N, Ito M.
    FEMS Microbiol. Lett. 341(1): 18-26 (2013)

2012年

  • Quality control of fungi-specific glucosylceramide in Cryptococcus neoformans by a novel glucocerebrosidase EGCrP1.
    Ishibashi Y, Ikeda K, Sakaguchi K, Okino N, Taguchi R, Ito M.
    J. Biol. Chem. 287(1): 368-381 (2012)
  • Mechanistic Insights into the 1,3-Xylanases: Useful Enzymes for Manipulation of Algal Biomass.
    Goddard-Borger E, Sakaguchi K, Reitinger S, Watanabe N, Ito M, Withers S.
    Journal of the American Chemical Society 134(8): 3895-3902 (2012)
  • A versatile transformation system for thraustochytrids applicable to both multiple transgene expression and gene targeting.
    Sakaguchi K, Matsuda T, Kobayashi T, Ohara J, Hamaguchi R, Abe E, Nagano N, Hayashi H, Ueda M, Honda D, Okita Y, TaokaY, Sugimoto S, Okino N, Ito M
    Applied and Environmental Microbiology 78(9): 3193-3202 (2012)
  • Gaining insight into the inhibition of glycoside hydrolase family 20 exo-β-N-acetylhexosaminidases using a structural approach.
    Sumida T, Stubbs KA, Ito M, Yokoyama S
    Org. Biomol. Chem. 10(13): 2607-2612 (2012)
  • The analysis of delta12 fatty acid desaturase function revealed that two distinct pathways are active for the synthesis of polyunsaturated fatty acids in Thraustochytrium aureum ATCC34304.
    Matsuda T, Sakaguchi K, Hamaguchi R, Kobayashi T, Abe E, Hama Y, Hayashi M, Honda D, Okita Y, Sugimoto S, Okino N, Ito M
    J. Lipid Res. 53(6): 1210-1222 (2012)
  • Loss-of-function Mutation in Bi-functional Marine Bacterial Sialyltransferase.
    Kajiwara H, Katayama S, Kakuta Y, Okino N, Ito M, Mine T, Yamamoto T
    Biosci. Biotechnol. Biochem. 76(9): 1639-1644 (2012)
  • Preparation and characterization of endoglycoceramdiase I, applicable to the comprehensive analysis of glycosphingolipids, using a rhodococcal expression system.
    Ishibashi Y, Kobayashi U, Hijikata A, Sakaguchi K, Goda H, Tamura T, Okino N, Ito M.
    J. Lipid Res. 53(10): 2242-2251 (2012)
  • Elevation of lipid peroxide level and production of hydroxy lipids in cultured Hepa-T1 cells by oxidative stressors.
    Tanaka R, Hatate H, Ito M, T Nakamura T
    Fisheries Science 72(3): 665-672 (2012)
  • Extracellular secretion of phenolic substances from living brown algae.
    Shibata T, Hama Y, Miyasaki T, Ito M, Nakamura T
    Applied Phycology 18(6): 787-794 (2012)

2011年

  • Qualitative and quantitative cellular glycomics of glycosphingolipids based on Rhodococcal EGCase -assisted glycan cleavage, glycoblotting-assisted sample preparation and MALDI-TOF/TOF MS analysis.
    Fujitani N, Takegawa Y, Ishibashi Y, Araki K, Furukawa JI, Mitsutake S, Igarashi Y, Ito M, Shinohara Y.
    J. Biol. Chem. 286(48): 41669-41679 (2011)
  • Detection of genes involved in fatty acid elongation and desaturation in thraustochytrid marine eukaryotes.
    Nagano N, Sakaguchi K, Taoka Y, Okita Y, Honda D, Ito M, Hayashi M.
    J. Oleo. Sci. 60(9): 475-81(2011)
  • The mucin box and signal/anchor sequence of rat neutral ceramidase recruit bacterial sphingomyelinase to the plasma membrane.
    Nakagawa T, Tani M, Sueyoshi N, Ito M.
    Biosci. Biotech. Biochem. 75(5): 987-990 (2011)
  • Glycosphingolipid receptor of mariculture fish intestines for phathogenic vibrios.
    Matsunaga N, Chisada S, Fujioka H, Takashima K, Okino N, Ito M.
    Fish. Sci. 77(4): 583-590 (2011)
  • Molecular cloning of a Pinguiochrysis pyriformis oleate-specific microsomal delta12-fatty acid desaturase and functional analysis in yeasts and thraustochytrids.
    Matsuda T, Sakaguchi K, Kobayashi T, Abe E, Kurano N, Sato A, Okita Y, Sugimoto S, Hama Y, Hayashi M, Okino N, Ito M.
    J. Biochem. 150(4): 375-383 (2011)
  • Lipid rafts enriched in monosialylGb5Cer carrying the stage-specific embryonic antigen-4 epitope are involved in development of mouse preimplantation embryos at cleavage stage.
    Sato B, Katagiri YU, Miyado K, Okino N, Ito M, Akutsu H, Okita H, Umezawa A, Fujimoto J, Toshimori K, Kiyokawa N.
    BMC Dev. Biol. Apr 14;11(1): 22 (2011)
  • Multiplex analysis of sphingolipids using amine-reactive tags (iTRAQ).
    Nabetani T, Makino A, Hullin-Matsuda F, Hirakawa TA, Takeoka S, Okino N, Ito M, Kobayashi T, Hirabayashi Y.
    J. Lipid Res. 52(6): 1294-302 (2011)
  • Increase of EPA in Thraustochytrids through Expression of a Fatty Acid Δ5 Desaturase Gene Driven by the Thraustochytrid Ubiquitin Promoter.
    Kobayashi T, Sakaguchi K, Matsuda T, Abe E, Hama Y, Hayashi M, Honda D, Okita Y, Sugimoto S, Okino N, Ito M.
    Appl. Environ. Microbiol. 77(11): 3870-6 (2011)
  • Ceramide glucosyltransferase of the nematode Caenorhabditis elegans is involved in oocyte formation and in early embryonic cell division.
    Nomura KH, Murata D, Hayashi Y, Dejima K, Mizuguchi S, Kage-Nakadai E, Gengyo-Ando K, Mitani S, Hirabayashi Y, Ito M, Nomura K.
    Glycobiology 21(6): 834-848 (2011)
  • Purification, molecular cloning, and application of a novel sphingomyelin-binding protein (clamlysin) from the brackishwater clam, Corbicula japonica.
    Takara T, Nakagawa T, Isobe M, Okino N, Ichinose S, Omori A, Ito M.
    Biochim. Biophys. Acta. 1811(5): 323-332 (2011)
  • Molecular cloning and catalytic mechanism of a novel glycosphingolipid-degrading β-N-acetylgalactosaminidase from Paenibacillus sp. TS12.
    Sumida T, Fujimoto K, Ito M.
    J. Biol. Chem. 286(16): 14065-14072 (2011)

2010年

  • β-galactosyltransferase-5 is a lactosylceramide synthase essential for mouse extra-embryonic development.
    Nishie T, Hikimochi Y, Zama K., Fukusumi Y, Ito M, Yokoyama H, Baruse C, Ito M, Asano M.
    Glycobiology 20(10): 1311-1322 (2010)
  • Lack of lacto/neolacto-glycolipids enhances the formation of glycolipid-enriched microdomains, facilitating B cell activation.
    Togayachi A, Kozono Y, Ikehara Y, Ito H, Suzuki N, Tsunoda Y, Abe S, Sato T, Nakamura K, Suzuki M, Goda HM, Ito M, Kudo T, Takahashi S, Narimatsu H.
    Proc. Natl. Acad. Sci. USA 107(26): 11900-11905 (2010)
  • Expression, purification, and characterization of a recombinant neutral ceramidase from Mycobacterium tuberculosis.
    Okino N, Ikeda R, Ito M.
    Biosci. Biotechnol. Biochem. 74(2): 316-321 (2010)

2009年

  • A novel fucosyl glycosphingolipid of brine shrimp that is highly sensitive to endoglycoceramidase.
    Xu X, Horibata Y, Inagaki M, Hama Y, Sakaguchi K, Okino N, and Ito M.
    Glycobiology 19(12): 1446-1451 (2009)
  • Zebrafish and mouse α2,3-sialyltransferases responsible for synthesizing GM4 ganglioside.
    Chisada S, Yoshimura Y, Sakaguchi K, Uchima H, Matsunaga N, Okino N, Uemura S, Go S, Ogura K, Tai T, Ikeda K, Taguchi R, Inokuchi J, and Ito M.
    J. Biol. Chem. 284(44): 30534-30546 (2009)
  • Molecular cloning and crystal structural analysis of a novel β-N-acetylhexosaminidase from Paenibacillus sp. TS12 capable of degrading glycosphingoliids.
    Sumida T., Ishii R, Yanagisawa T., Yokoyama S., and Ito M.
    J. Mol. Biol. 392(1): 87-99 (2009)
  • Characterization and application of carbohydrate-binding modules of β-1,3-xylanase XUL4.
    Kiyohara M, Sakaguchi K, Yamaguchi K, Araki T, and Ito M.
    J. Biochem. 146(5): 633-641 (2009)
  • Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP) is indispensable for normal embryogenesis in zebrafish, Danio rerio.
    Sueyoshi N, Nimura T., Ishida A, Taniguchi T, Yoshimura Y, Ito M, Shigeri Y, and Kameshita I.
    Arch. Biochem. Biophys. 488(1): 48-59 (2009)
  • Crystal structure of α/β-galacoside α2,3-sialyltransferase from a luminous marine bacterium, Photobacterium phosphoreum.
    Iwatani T., Okino N., Sakakura M, Kajiwara H., Takakura Y., Kimura M, Ito M, Yamamoto Y, and Kakuta Y.
    FEBS Lett. 583(12): 2083-2087 (2009)
  • Transglycosylation-based Fluorescent Labeling of 6-Gala Series Glycolipids by EGALC.
    Ishibashi Y, Nagamatsu Y, Meyer S, Imamura A, Ishida H, Kiso M, Okino N, Geyer R, Ito M.
    Glycobiology 19(7): 797-807 (2009)
  • Simultaneous quantification of glucosylceramide and galactosylceramide by normal-phase HPLC using O-phtalaldehyde derivatives prepared with sphingolipid ceramide N-deacylase.
    Zama K, Hayashi Y, Ito Y, Hirabayashi Y, Inoue T, Ohno K, Okino N, Ito M.
    Glycobiology 19(7): 767-775 (2009)
  • Tamavidins--novel avidin-like biotin-binding proteins from the Tamogitake mushroom.
    Takakura Y, Tsunashima M, Suzuki J, Usami S, Kakuta Y, Okino N, Ito M, Yamamoto T.
    FEBS J. 276(5): 1383-1397 (2009)
  • Mechanistic insights into the hydrolysis and synthesis of ceramide by neutral ceramidase.
    Inoue T, Okino N, Kakuta Y, Hijikata A, Okano H, Goda HM, Tani M, Sueyoshi N, Kambayashi K, Matsumura H, Kai Y, Ito M.
    J. Biol. Chem. 284(14): 9566-9577 (2009)

2008年

  • A sensitive and reproducible fluorescent-based HPLC assay to measure the activity of acid as well as neutral β-glucocerebrosidases.
    Hayashi Y, Zama K, Abe E, Okino N, Inoue T, Ohno K, Ito M.
    Analytical Biochemistry 383: 122-129 (2008)
  • Molecular cloning, expression, and characterization of a novel endo-α-N-acetylgalactosaminidase from Enterococcus faecalis.
    Goda HM, Ushigusa K, Ito H, Okino N, Narimatsu H, Ito M.
    Biochemical and Biophisical Reserch Communications 375: 541-546 (2008)
  • Crystal structure of the covalent intermediate of human cytosolic β-glucosidase.
    Noguchi J, Hayashi Y, Baba Y, Okino N, Kimura M, Ito M, Kakuta Y.
    Biochemical and Biophisical Reserch Communications 374: 549-552 (2008)
  • Crystal Structure of Vibrionaceae Photobacterium sp. JT-ISH-224 α2,6-Sialyltransferase in a Ternary Complex With Donor Product CMP and Acceptor Substrate Lactose.
    Kakuta Y, Okino N, Kajiwara H, Ichikawa N, Takakura Y, Ito M, Yamamoto Y.
    Glycobiology 18: 66-73 (2008)

 

2007年

  • Purification, crystallization and preliminary crystallographic characterization of the alpha 2,6-sialyltransferase from Photobacterium sp. JT-ISH-224.
    Okino N, Kakuta Y, Kajiwara H, Ichikawa M, Takakura Y, Ito M, Yamamoto T
    Acta Crystallogr Sect F Struct Biol Cryst Commun. 63: 662-664 (2007)
  • Synthesis of fluorescent glycosphingolipids and neoglycoconjugates which contain 6-gala oligosaccharides using the transglycosylation reaction of a novel endoglycoceramidase (EGALC).
    Ishibashi Y, Kiyohara M, Okino N, Ito M
    J. Biochem. 142: 239-246 (2007)
  • Klotho-related protein is a novel cytosolic neutral glycosylceramidase.
    Hayashi Y, Okino N, Kakuta Y, Shikanai T, Tani M, Narimatsu H, Ito M
    J. Biol. Chem. 282: 30889-30900 (2007)
  • Ceramidase enhances phospholipase C-induced hemolysis by Pseudomonas aeruginosa.
    Okino N, Ito M
    J. Biol. Chem. 282: 6021-6030 (2007)
  • A Novel Endoglycoceramidase hydrolyzes oligogalactosylceramides to produce galactooligosaccharides and ceramides.
    Ishibashi Y, Nakasone T, Kiyohara M, Horibata Y, Sakaguch K, Hijikata A, Ichinose S, Omori A, Yasui Y, Imamura A, Ishida H, Kiso M, Okino N, Ito M
    J. Biol. Chem. 282: 11386-11396 (2007)
  • Fucosyl-GM1a, an endoglycoceramidase-resistant ganglioside of porcine brain.
    Xu X, Monjusho H, Inagaki M, Hama Y, Yamaguchi K, Sakaguchi K, Iwamori M, Okino N, Ito M
    J. Biochem. 141: 1-7 (2007)
  • Ceramide/sphingosine/sphigosine 1-phosphate metabolism on the cell surface and in the extracellular space.
    Tani M, Ito M, Igarashi Y
    Cell Signal. 19: 229-237 (2007)
  • Knock down of nuclear Ca2+/calmodulin-dependent protein kinase phosphatase cause developmental abnormalities in zebrafish.
    Nimura T, Sueyoshi N, Ishida A, Yoshimura Y, Ito M, Tokumitsu H, Shigeri Y, Nozaki N, Kameshita I
    Arch. Biochem. Biophys. 457: 205-216 (2007)
  • Ganglioside from eel serum high density lipoprotein (HDL) and its role as a ligand for HDL binding protein.
    Kumagai A, Chisada S, Kamei Y, Ito S, Hayashi S
    Comp. Biochem. Physiol. B Biochem. Mol. Biol. 147(4): 635-644 (2007)

2006年

  • Structure of β-1,3 Xyloologosaccharide Generated from Caulerpa racemosa var. laete-virens β-1,3-Xylan by the Action of β-1,3-Xylanase.
    Kiyohara M, Hama Y, Yamaguchi K, Ito M
    J. Biochem. 140: 369-373 (2006)
  • A Novel Phosphatidylcholine Which Contains Pentadecanoic Acid at sn-1 and Docosahexaenoic Acid at sn-2 in Schizochytrium sp. F26-b.
    Abe E, Hayashi Y, Hama Y, Hayashi M, Inagaki M, Ito M
    J. Biochem. 140: 247-253 (2006)
  • Escherichia coli thereby establishing the usefulness of the SUNGA method in exploring the function of N-glycans in vivo. Substitution of the N-glycan function in glycosyltransferases by specific amino acids: ST3Gal-V as a model enzyme.
    Uemura S, Kurose T, Suzuki T, Yoshida S, Ito M, Saito M, Horiuchi M, Inagaki F, Igarashi Y, Inokuchi J
    Glycobiology 19: 258-270 (2006)
  • Exracellular secretion of phenolic substacnes from living browon algae.
    Shibata T, Hama Y, Miyasaki M, Ito M, Nakamura T
    J. Appl. Physiol. 18: 787-794 (2006)
  • Elecation of lipid peroxide level and production of hydroxy lipids in cultured Hepa-T1 cells by oxidative stresors.
    Tanaka R, Hatate H, Ito M, Nakamura T
    Fish. Science 72: 665-672 (2006)

 

2005年

  • Involvement of neutral ceramidase in ceramide metabolism at the plasma membrane and in extracellular milieu.
    Tani M, Igarashi Y, Ito M
    J. Biol. Chem. 280: 36592-36600 (2005)
  • Mechanism of sphingosine and sphingosine 1-phosphate generation in human platelets.
    Tani M, Sano T, Ito M, Igarashi Y
    J. Lipid Res. 46: 2458-2467 (2005)
  • A sensitive and reproducible assay to measure the activity of glucosylceramide synthase and lactosylceramide synthase using HPLC and fluorescent substrates.
    Hayashi Y, Horibata Y, Sakaguchi K, Okino N, Ito M
    Anal. Biochem. 345: 181-186 (2005)
  • The glycosphingolipid receptor fro Vibrio trachuri in the red sea bream intestine is a GM4 ganglioside which contains 2-hydrosxy fatty acids.
    Chisada S, Horibata Y, Hama Y, Inagaki M, Furuya N, Okino N, Ito M
    Biochem. Biophys. Res. Commun. 333: 367-373 (2005)
  • Facile method for the preparation of lyso-GM1 and lyso-GM2.
    Ando T, Li SC, Ito M, Li YT
    J. Chromato. A 1078: 193-195 (2005)
  • Subcellular localization of human neutral ceramidase expressed in HEK293 cells.
    Hwang YH, Tani M, Nakagawa T, Okino N, Ito M
    Biochem. Biophys. Res. Commun. 331: 37-42 (2005)
  • Alpha-linolenic acid-containing GM1a induces apoptosis in Neuro2a cells: Application of SCDase for remodeling of fatty acids of glycosphingolipids.
    Nakagawa T, Morotomi A, Tani M, Sueyoshi N, Komori H, Ito M
    J. Lipid Res. 46: 1103-1112 (2005)
  • Molecular cloning and characterization of a novel β-1,3-xylanase possessing two putative carbohydrate-binding modules from a marine bacterium Vibrio sp. strain AX-4.
    Kiyohara M, Sakaguchi K, Yamaguchi K, Araki T, Nakamura T, Ito M.
    Biochem. J. 388: 949-957 (2005)

 

2004年

  • Molecular cloning and functional analysis of zebrafish neutral ceramidase.
    Yoshimura Y, Tani M, Okino N, Iida H, Ito M
    J. Biol. Chem. 279: 44012ー44022 (2004)
  • Unique catabolic pathway of glycosphingolipids in a hydrozoan, Hydra magnipapillata, involving endoglycoceramidase.
    Horibata Y, Sakaguchi K, Okino N, Iida H, Inagaki M, Fujisawa T, Hama Y, Ito M.
    J. Biol. Chem. 279: 33379-33389 (2004)
  • Conserved amino acid residues in the COOH-terminal tail are indispensable for the correct folding and localization, and enzyme activity of neutral ceramidase.
    Tani M, Okino N, Sueyoshi N, Ito M
    J. Biol. Chem. 279: 29351-29358 (2004)
  • Paenibacillus sp. TS12 glucosylceramidase: kinetic studies of a novel sub-family of family 3 glycosidases and identification of the catalytic residues.
    Paal K, Ito M, Withers SG
    Biochem. J. 378: 141-149 (2004)
  • Preparation and preliminary X-ray analysis of the catalytic module of β-1,3-xylanase from the marine bacterium Vibrio sp. AX-4.
    Sakaguchi K, Kiyohara M, Watanabe N, Yamaguchi K, Ito M, Kawamura T, Tanaka I.
    Acta Cryst. D60: 1470-1472 (2004)
  • Application of RNA interference to chiken embryos using small interfering RNA.
    Sato F, Nakagawa T, Ito M, Kitagawa Y, Hattori M
    J. Exp. Zool. 301A: 820-827 (2004)

 

2003年

  • A neutral ceramidase homologue of Dictyostelium discoideum exhibits an acidic pH optimum.
    Monjusho H, Okino N, Tani M, Maeda M, Yoshida M, Ito M
    Biochem. J. 376: 473-479 (2003)
  • Purification and characterization of recombinant, human acid ceramidase: Catalytic reactions and interactions with acid sphingomyelinase.
    He X, Okino N, Dhami R, Dagan A, Gatt S, Schulze H, Sandhoff K, Schuchman EH
    J. Biol. Chem. 278: 32978-32986 (2003)
  • The reverse activity of human acid ceramidase.
    Okino N, He X, Gatt S, Sandhoff K, Ito M, Schuchman EH
    J. Biol. Chem. 278: 29948-29953 (2003)
  • O-Glycosylation of mucin-like domain retains the neutral ceramidase on the plasma membranes as a type II integral membrane protein.
    Tani M, Iida H, Ito M
    J. Biol. Chem. 278: 10523-10530 (2003)
  • Neutral ceramidase secreted by endotherlial cells is released in part associated with caveolin-1.
    Romiti E, Meacci E, Donati C, Formigli L, Zecchi-Orlandini S, Farnararo M, Ito M, Bruni P
    Arch. Biochem. Biophy. 417: 27-33 (2003)

 

2002年

  • Genomic structure and promoter analysis of the mouse neutral ceramidase gene.
    Okino N, Mori K, Ito M
    Biochem. Biophys. Res. Commun. 299: 160-166 (2002)
  • Molecular cloning and characterization of sphingolipid ceramide N-deacylase from a marine bacterium, Shewanella alga G8.
    Furusato M, Sueyoshi N, Mitsutake S, Sakaguchi K, Kita K, Okino N, Ichinose S, Omori A, Ito M
    J. Biol. Chem. 277: 17300-17307 (2002)
  • Purification, characterization, and cDNA cloning of a novel N-acetylgalactosamine-specific lectin from starfish, Asterina pectinifera.
    Kakiuchi M, Okino N, Sueyoshi N, Ichinose S, Omori A, Kawabata S, Yamaguchi K, Ito M.
    Glycobiology 12: 85-94 (2002)
  • Activation of bacterial ceramidase by anionic glyceropholpholipids. - Possible involvement in ceramide hydrolysis on atopic skin by bacterial ceramidase –
    Kita K, Sueyoshi N, Okino N, Inagaki M, Ishida H, Kiso M, Imayama S, Nakamura T, Ito M
    Biochem. J. 362: 619-626 (2002)
  • Molecular Cloning and Expression of Mn2+-Dependent Sphingomyelinase/Hemolysin of an Aquatic Bacterium, Pseudomonas sp. TK4.
    Sueyoshi N, Kita K, Okino N, Sakaguchi K, Nakamura T, Ito M.
    J. Bacteriol. 184: 540-546 (2002)
  • Utilization of ganglioside-degrading Paenibacillus sp. strain TS12 for production of glucosylceramide.
    Sumida T, Sueyoshi N, Ito M
    Appl. Environmental Microbiology 68: 5241-5248 (2002)
  • Molecular cloning and characterization of a novel glucocerebrosidase of Paenibacillus sp.
    Sumida T, Sueyoshi N, Ito M
    J. Biochem. 132: 237-243 (2002)
  • Molecular cloning and characterization of a secretary neutral ceramidase of Drosophila melanogaster.
    Yoshimura Y, Okino N, Tani M, Ito M
    J. Biochem. 132: 229-237 (2002)
  • Possible involvement of Pseudomonas sphingolipid ceramide N-deacylase in red spot disease of eels.
    Kita K, Sakaguchi K, Furuya N, Iida T, Ito M
    J. Fac. Agr. Kyushu Univ. 46: 331-338 (2002)
  • Mutation analysis of the acid ceramidase gene in Japanese patients with Farber disease.
    Muramatus T, Sakai N, Yanagihara L, Yamada M, Nishigaki T, Ito M, Inui K
    J. Inherit. Metab. Dis. 25: 585-592 (2002)
  • Inhibitory activity of brown algal phlorotannins against hyaluronidase.
    Shibata T, Fujimoto K, Yamaguchi K, Nagayama K, Nakamura T
    Inter. J. Food Sci. Tech. 37: 703-709 (2002)
  • Inhibitory activity of brown algal phlorotannins against glycosidases from the viscera of the turban shell Turbo cornutus.
    Shibata T, Yamaguchi K, Nagayama K, Kawaguchi S, Nakamura T
    Eur. J. Phycology 37: 493-500 (2002)

2001年

  • Apoptosis of Neuro2a cells induced by lysosphingolipids with naturally occurring stereochemical configurations.
    Sueyoshi N, Maehara T, Ito M
    J. Lipid Res 42: 1197-1202 (2001)
  • Inhibitory activity of sulfoglycolipid derivatives towards pancreatic trypsin
    Suzuki H, Ito M, Kimura Y, Iwamor Y, Iwamori M
    Glycoconjugate Journal 17: 787-793 (2001)
  • Transglycosilation and Reverse Hydrolysis Reaction of Endoglycoceramidase from the Jellyfish, Cyanea nozakii
    Horibata Y, Higashi H, Ito M.
    J. Biohem. 130: 263-268 (2001)
  • Purification, characterization, molecular cloning and subcellular distribution of neutral ceramidase of rat kidney
    Mitsutake S, Tani M, Okino N, Iida H, Yoshida S, Omori A, Nakamura T, Ito M
    J. Biol. Chem. 276: 26249-26359 (2001)
  • Distribution of neutral/alkaline ceramidase in marine invertebrates revealed by fluorescence-labeld ceramide
    Nakagawa T, Ito M.
    Fish Science 67: 544-546 (2001)
  • Localization of neutral ceramidase in caviolin-enriched light membranes of murine endothelial cells.
    Romit E, Meacci E, Tanzi G, Becciolini L, Mitsudake S, Famarano M, Ito M, Bruni P.
    FEBS Lett. 506: 163-168 (2001)
  • Characterization of the reversible nature of the reaction catalyzed by sphingolipid ceramide N-deacylase -A NOVEL FORM OF REVERSE HYDROLYSIS REACTION-
    Kita K, Kurita T, to M
    Eur. J. Biochem. 268: 592-602 (2001)

2000年

  • Enhancement of hydrolytic activity of sphingolipid ceramide N-deacylase in the aqueous organic biphasic system.
    Kurita T, Izu H, Sano M, Ito M, Kato I.
    J. Lipid Res 41: 846-851 (2000)
  • Purification, characterization and cDNA cloning of a novel acidic endoglycoceramidase from the jellyfish, Cyanea nozakii.
    Horibata Y, Okino N, Ichinose S, Omori A, Ito M.
    J. Biol. Chem. 275: 31297-31304 (2000)
  • Regulation of UDP-glucose:ceramide glucosyltransferase-1 by ceramide.
    Komori H, Ichinose S, Hirabayashi Y, Ito M.
    FEBS letter 475: 247-250 (2000)
  • Reverse hydrolysis reaction of recombinant alkaline ceramidase of Pseudomonas aeruginosa.
    Kita K, Okino N, Ito M.
    Biochim. Biophys. Acta. 1485: 111-120 (2000)
  • Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase. - A novel but highly conserved gene family of neutral/alkaline ceramidases- .
    Tani M, Okino N, Mori K, Tanigawa T, Izu H, Ito M.
    J. Biol. Chem. 275: 11229-11234 (2000)
  • Purification and characterization of a neutral ceramidase from mouse liver. -A single protein catalyzes the reversible reaction in which ceramide is both hydrolyzed and synthesized- .
    Tani M, Okino N, Mitsutake S, Tanigawa T, Izu H, Ito M.
    J. Biol. Chem. 275: 3462-3468 (2000)
  • Cloning and expression of gene encoding a novel endoglycoceramidase of Rhodococcus sp. strain C9.
    Sakaguchi K, Okino N, Sueyoshi N, Izu H, Ito M.
    J. Biochem. 128: 145-152 (2000)
  • Neutral/alkaline and acid ceramidase activities are actively released by murine endothelial cells.
    Romiti E, Meacci E, Tani M, Nuti F, Farnararo M, Ito M, Bruni P.
    Biochem. Biophy. Res. Commun. 275: 746-751 (2000)
  • Isolation and characterization of collagen from rhizotomous jellyfish (Rhopilema asamushi).
    Nagai T, Worawattanamaeekul W, Suzuki T, Nakamura T, Ito T, Fujiki K, Nakao M, Yano T.
    Food Chemistry 70: 205-208 (2000)
  • Stereochemical assignment of cinjugated carbonyls with a prostaglandin-like ring structureformed during autoxidation of linolenate.
    Hama Y, Mastui T, Hatate H, Nakamura T.
    J. Jpn. Oil Chem. Soc. 49(8): 831-837 (2000)

1999年

N. Okino, S. Ichinose, A. Omori, S. Imayama, T. Nakamura, and M. Ito: Molecular cloning, sequencing, and expression of the gene encoding alkaline ceramidase from Pseudomonas aeruginosa. -Cloning of a ceramidase homologue from Mycobacterium tuberculosis- J. Biol. Chem. 274, 36616-36622 (1999)

M. Ito, K. Kita, T. Kurita, N. Sueyoshi, and H. Izu: Enzymatic N-deacylation of sphingolipids. Method in Enzymology 311, 297-303 (1999)

M. Ito, S. Mitusutake, M. Tani, and K. Kita: Enzymatic synthesis of 14C-ceramide, 14C-glycosphingolipids and w-aminoceramide. Method in Enzymology 311, 682-689 (1999)

H. Komori, S. Ichikawa, Y. Hirabayashi, and M. Ito: Regulation of intracellular ceramide content in B16 melanoma cells. -Biological implications of ceramide glycosylation- J. Biol. Chem. 274, 8981-8987 (1999)

M. Tani, N. Okino, S. Mitsutake, and M. Ito: Specific and sensitive assay for alkaline and neutral ceramidases involving C12-NBD-ceramide. J. Biochem. 125, 746-749 (1999)

K. Sakaguchi, N. Okino, H. Izu, and M. Ito: The Glu residue in the conserved Asn-Glu-Pro sequence of endoglycoceramidase is essential for enzymatic activity. Biochem. Biophy. Res. Commun. 260, 89-93, （1999）

T. Nakagawa, M. Tani, K. Kita, and M. Ito: Synthesis of fluorescent GM1 and SM utilizing the reverse hydrolysis reaction of sphingolipid ceramide N-deacylase: Use of fluorescent substrates for assays of sphingolipid degrading enzymes and sphingolipid-binding proteins. J. Biochem. 126, 111-119 (1999)

Y. Ohnishi, N. Okino, M. Ito, and S. Imayama: Ceramidase activity in bacterial skin flora as a possible cause of ceramide deficiency in atopic dermatitis. Clinic. Diagnos. Lab. Immun. 6, 101-104 (1999)

T. Nagai, T. Ogawa, T. Nakamura, T. Ito, H. Nakagawa, K. Fujiki, M. Nakao, and T. Yano: Collargen of edible jellyfish exumbrella. J. Sci. Food Agric. 79, 855-858 (1999).

T. Nagai, M. Kurata, T. Nakamura, T. Ito, K. Fujiki, M. Nakao, and T. Yano: Properties of myofibrillar protein from Japanese stingfish(Sebastes intermis) dorsal muscle. Food Research International 32, 401-405(1999)

R. Tanaka, Y. Higo, H. Murata, and T. Nakamura: Accumulation of hydroxy lipids in live fish with oxidative stress. Fisheries Science 65(5), 567-568 (1999).

Y. Miura, T. Arai, M. Ito, K. Yamamoto, and T. Yamagata; Different prerepuisite of hydrophobic moiety and a reliable chromogenic substrate for endo-type glycosylceramidases. Glycobiology 9, 957-960 (1999)

1998年

N. Okino, M. Tani, S. Imayama, and M. Ito: Purification and characterization of a novel ceramidase from Pseudomonas aeruginosa. J. Biol. Chem. 273,14368-14373 (1998)

M. Tani, K. Kita, H. Komori, T. Nakagawa, and M. Ito: Enzymatic synthesis of w-amino-ceramide: preparation of a sensitive fluorescent substrate for ceramidase. Anal. Biochem. 263, 183-188 (1998)

S. Mitsutake, K. Kita, T. Nakagawa, and M. Ito: Enzymatic synthesis of [14C]-glycosphingolipids by reverse hydrolysis reaction of sphingolipid ceramide N-deacylase: Detection of EGCase activity in a seaflower. J. Biochem. 123, 859-863 (1998)

L. Ji, M. Ito, G. Zhang, Y. Hirabayashi, J. Inokuchi, and T. Yamagata: Effects of endoglycoceramidase or D-threo PDMP on glucose uptake, glycolysis and mitochondrial respiration in HL60 cells. Arch. Biochem. Biophys. 359, 107-114 (1998)

J. Mitoma, M. Ito, S. Furuya, and Y. Hirabayashi: Bipotential roles of ceramide in the growth of hippocampal neurons: Promotion of cell survival and dendritic outgrowth in dose- and　developmental stage-dependent manners. J. Neurosci. Res. 51, 712-722 (1998)

M. Owon, K. Taira, and T. Nakamura: Separation and characterization of essential and fixed oil of black cumin (Nigella sativa L.) seeds. J. Agric. Res. Tanta Univ., 24(2), 172-182(1998)

T. Nakamura, R. Tanaka, Y. Higo, K. Taira, and T. Takeda: Lipid Peroxide Levels in tissues of Live Fish. Fisheries Science 64(4), 617-620（1998）

T. Nakamura, Y. Hama, R. Tanaka, K. Taira, and H. Hatade: A new red coloration induced by the reaction of oxidized lipids with amino acids. J. Agric. Food Chem. 46(4), 1316-1320（1998）

K. Yamaguchi, Y. Izumi, and M. Kitamikado: Purification and Characterization of β-Xylosidase from Bacillus sp. XY-131. Fisheries Science 64 (5) 787-792 (1998）

1997年

N. Sueyoshi, H. Izu, and M. Ito: Preparation of a naturally occurring D-erythro-(2S,3R)-sphingosylphosphorylcholine using Shewanella alga NS-589. J. Lipid Res., 38,1923-1927 (1997)

H. Izu, Y. Izumi, Y. Kurome, M. Sano, A. Kondo, I. Kato, and M. Ito: Molecular cloning, expression, and sequence analysis of the endoglycoceramidase II gene from Rhodococcus sp. strain M-777. J. Biol. Chem. 272, 19846-19850 (1997)

Y. Fukano, and M. Ito: Preparation of GM1 ganglioside using silidase-producing marine bacteria as a microbial biocatalyst. Appl. Environ. Microbiology 63, 1861-1865 (1997)

S. Mitsutake, K. Kita, N. Okino, and M. Ito: Synthesis of 14C-labeled ceramide by fatty acid transfer reaction of sphigolipid ceramide N-deacylase and its application for assay of ceramidase. Anal. Biochem. 247, 52-57 (1997)

1996年

M. Ito, and H. Komori: Homeostasis of cell-surface glycosphingolipid content in B16 melanoma cells.-Evidence revealed by an endoglycoceramidase- J. Biol. Chem. 271, 12655-12660 (1996)

T. Nakamura, M. Fukuda, and R. Tanaka: A simple method for identification of lipids from aquatic organisms using thin-layer chromatography on a plainsilica gel plate. Lipids 31(4), 427-432 (1996)

T. Nakamura, K. Nagayama, K. Uchida, and R. Tanaka: Antioxidant activity of phlorotannins isolated from the brown alga Eisenla bycyclis. Fish. Science 62(6), 923-926(1996)

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    生化学 90(2):178-182 (2018)
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  • Neutral Ceramidase.
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    Encyclopedia of Signaling Molecules. 1-8 (2016)
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    Trends in Glycoscience and Glycotechnology vol.28 No.164, E117-118, J115-116, 2016
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    Vitam. Horm. 101:1-16 (2016)
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    バイオサイエンスとインダストリー Vol. 72 (2014)
  • New insight into the structure, reaction mechanism, and biological functions of neutral ceramidase.
    Ito M, Okino N, Tani M.
    Biochim. Biophys. Acta. 1841(5): 682-691 (2014)
  • ラビリンチュラ類の分子育種の基盤構築と高度不飽和脂肪酸生合成経路の解明
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    バイオサイエンスとインダストリー　Vol. 71 (2013)
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    Ishibashi Y, Kohyama-Koganeya A, Hirabayashi Y.
    Biochim, Biophys. Acta. 1831(9):1475-1485(2013)
  • クリプトコッカス症原因菌における糖脂質の代謝・品質管理機構の解明
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    日本応用酵素協会誌 No. 48(2013)
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    生化学、82(1): 47-53 (2010)
  • 細菌セラミダーゼの生理機能と高次構造の解明
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    生化学、81(10): 911-916 (2009)
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  • Neutral ceramidase as an integral modulator for the generation of S1P and S1P-mediated signaling.
    Ito M, Tani M, Yoshimura Y
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